食品科学

• 基础研究 • 上一篇    下一篇

浓缩苹果汁生产过程中cAMP分布规律研究

冉仁森,郭玉蓉,刘永峰*,仇农学,牛鹏飞   

  1. 陕西师范大学食品工程与营养科学学院,陕西 西安 710062
  • 出版日期:2014-05-15 发布日期:2014-05-19

Distribution of cAMP during Production of Concentrated Apple Juice

RAN Ren-sen, GUO Yu-rong, LIU Yong-feng*, QIU Nong-xue, NIU Peng-fei   

  1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China
  • Online:2014-05-15 Published:2014-05-19

摘要:

探究浓缩苹果汁生产过程中3’,5’-环磷酸腺苷(3’,5’-cyclic adenosine monophosphate,cAMP)含量的分布规律。利用HPLC方法测定水浸提和乙醇浸提两种不同方式处理榨汁后的苹果果渣的cAMP含量和浓缩果汁中cAMP的含量,分析不同稀释倍数、不同可溶性固形物含量的浓缩苹果汁cAMP变化规律,并利用cAMP分配系数(DCcAMP)描述榨汁过程中cAMP含量的分布规律。结果表明:榨汁后苹果果渣中cAMP含量为(63.444 1±2.323 6)μg/g,70 °Brix的浓缩苹果汁成品中cAMP含量为(58.611 3±1.349 3)μg/mL,30 °Brix的浓缩苹果汁半成品中cAMP含量为(12.018 3±0.583 9)μg/mL;建立了浓缩苹果汁中cAMP含量与可溶性固形物含量之间的关系方程为y= 0.808 5x-1.218 5;测定得到70 °Brix和30 °Brix浓缩苹果汁的DCcAMP分别为92%和19%。因此,在浓缩苹果汁生产过程中,cAMP更趋向于存在苹果果渣中,且浓缩工艺对cAMP有富集作用。

关键词: 浓缩苹果汁, 苹果果渣, cAMP, 高效液相色谱

Abstract:

The distribution pattern of 3’,5’-cyclic adenosine monophosphate(cAMP) during the production
of concentrated apple juice was investigated. HPLC was employed to determine the cAMP content in
apple pomace left after juice extraction with water or aqueous ethanol and in concentrated apple juice. The
change trend of cAMP content in concentrated apple juice was analyzed as a function of dilution factor and
soluble solid content. Distribution coefficient (DCcAMP) was calculated to describe the distribution regularity
of cAMP. The result showed that cAMP contents in apple pomace, 70 °Brix concentrated apple juice, and
30 °Brix concentrated apple juice were (63.444 1 ± 2.323 6) μg/g, (58.611 3 ± 1.349 3) μg/mL and (12.018 3 ± 0.583 9) μg/mL,
respectively. An equation indicating cAMP content (y) as a function of soluble solid content (x) in concentrated apple juice
was established as follows: y = 0.808 5x − 1.218 5. Furthermore, the DCcAMP of 70 °Brix and 30 °Brix concentrated apple
juice were 92% and 19%, respectively. Thus, during the production of concentrated apple juice, cAMP preferred to exist in
apple pomace and could be enriched by the concentration process

Key words: apple juice concentrate, apple pomace, 3’,5’-cyclic adenosine monophosphate, HPLC