食品科学

• 基础研究 • 上一篇    下一篇

壳聚糖-乳清分离蛋白复合膜的制备、形态结构及理化性质

陈 悦,陈季旺,周 琦,夏文水   

  1. 1.武汉轻工大学食品科学与工程学院,湖北 武汉 430023;2.农产品加工湖北省协同创新中心,湖北 武汉 430023;
    3.江南大学食品学院,江苏 无锡 214122
  • 出版日期:2014-05-15 发布日期:2014-05-19

Preparation, Morphological Structure, and Physico-chemical Properties of Chitosan-Whey Protein Isolate Composite Film

CHEN Yue, CHEN Ji-wang, ZHOU Qi, XIA Wen-shui   

  1. 1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 2. Hubei Collaborative Innovation Center for Processing of Agricultural Products, Wuhan 430023, China; 3. School of Food Science and Technology,Jiangnan University, Wuxi 214122, China
  • Online:2014-05-15 Published:2014-05-19

摘要:

以壳聚糖(chitosan,CTS)和乳清分离蛋白(whey protein isolate, WPI)为成膜基质,制备壳聚糖-乳清分离蛋白复合膜(chitosan/whey protein isolate composite film,CWF),并分析CWF的理化性质。通过测定CWF的拉伸强度、断裂延伸率、水蒸气透过率、透明度,优化CWF的成膜条件为CTS脱乙酰度90%、分子质量300 kD,成膜液pH 3,甘油添加量1.5%,WPI添加量0.5%。CWF的机械性能和剥离性比CTS膜显著改善,WVP和透明度有良好的改善。扫描电镜分析显示CWF的横截面更规则、均匀,且外观为均匀半透明膜。傅里叶红外光谱扫描结果显示CTS、WPI制备CWF时在其分子之间形成了强烈的相互作用,二者有良好的相容性。

关键词: 壳聚糖-乳清分离蛋白复合膜, 理化性质, 扫描电镜, 红外光谱仪

Abstract:

In this present work, chitosan-whey protein isolate composite film (CWF) was prepared by casting technique
through mixing chitosan (CTS) dissolved in 2% (by volume) aqueous acetic acid with 1 g/100 mL whey protein isolate
(WPI) dissolved in deionized water under continuous stirring. The tensile strength (TS), breaking elongation rate (E), water
vapor permeability (WVP), and transparency of CWF were determined. The preparation conditions of CWF were optimized
to be CTS with a deacetylation degree (DD) of 90% and a molecular weight (MW) of 300 kD in a film-forming solution at
pH 3 with the addition of 1.5% glycerol and 0.5% WPI aqueous solution. The mechanical properties and stripping
property of CWF were significantly improved over those of CTS, and CWF also showed a slight improvement in WPI and
transparency. Scanning electron microscopy (SEM) analysis indicated that the cross-sectional microstructure of CWF was
uniform and compact, and the appearance was semi-translucent. Fourier transform infrared spectrometer (FTIR) results
showed a strong interaction and good compatibility between the molecules of CTS and WPI in the preparation of CWF.

Key words: chitosan-whey protein isolate composite film, physico-chemical properties, scanning electron microscopy (SEM);fourier transform-infrared spectrometer (FT-IR)