食品科学

• 生物工程 • 上一篇    下一篇

杏鲍菇污染菌的分离、鉴定及其特性

姚璐晔,沈金波,戴 璐,裴丹丹,孙丹凤,冀 宏   

  1. 常熟理工学院生物与食品工程学院,江苏 常熟 215500
  • 出版日期:2014-05-15 发布日期:2014-05-19

Isolation, Identification and Characterization of Contaminant Bacteria from Pleurotus eryngii

YAO Lu-ye, SHEN Jin-bo, DAI Lu, PEI Dan-dan, SUN Dan-feng, JI Hong   

  1. School of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Online:2014-05-15 Published:2014-05-19

摘要:

从夏季被细菌污染的杏鲍菇上分离纯化得到2 株病害菌(CTE722-B、CTE722-C),结合显微结构和生理生化实验结果,以及对它们16S rDNA序列的分析确定这两株菌的系统发育地位;通过抑菌圈实验考察几种工厂常用消毒剂对病害菌的抑菌影响。结果表明:菌株CTE722-B鉴定为恶臭假单胞菌属(Pseudomonas putida),CTE722-C为边缘假单胞菌属(Pseudomonas marginalis)。消毒剂菇保、甲酚皂、苯扎溴铵消毒液在短时间内对这两种细菌都有较好的抑菌效果;但因其具有挥发性,故防治效果不理想。从污染菇体分离得到的2 株病害菌CTE722-B和CTE722-C被鉴定为假单胞菌属,因常用消毒剂只在短时间内对其有抑菌性,防治效果较差。

关键词: 杏鲍菇, 病害菌, 假单胞菌, 特性

Abstract:

This study aimed to identify and characterize the pathogenic bacteria which caused pollution in Pleurotus eryngii.
Two strains named CTE722-B and CTE722-C were isolated from Pleurotus eryngii polluted in summer collected from a
mushroom factory. According to their microstructure, physiological, biochemical properties and 16S rDNA sequences,
the phylogenetic positions of the two strains were defined. By measuring the diameter of inhibition zone, the efficacy of
sanitizers commonly used in the food industry against them was investigated. The results showed that CTE722-B and
CTE722-C were identified as Pseudomonas putida and Pseudomonas marginais, respectively. Although the growth of two
strains was restrained within a short time after adding dichloroisocyanuric acid sodium salt, cresol soap or benzalkonium
bromide into the medium, the antibacterial efficacy of these sanitizers was poor due to the high volatility.

Key words: Pleurotus eryngii, pathogenic bacteria, Pseudomonas, characteristics