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响应面法优化枯草芽孢杆菌S0507产四甲基吡嗪的培养条件

祝赛峰,吴建峰,赵希荣   

  1. 1.淮阴工学院生命科学与化学工程学院,江苏 淮安 223003;2.江苏今世缘酒业有限公司,江苏 涟水 223411
  • 出版日期:2014-05-15 发布日期:2014-05-19

Response Surface Methodology for Optimization of Culture Conditions for the Production of Tetramethylpyrazine by Bacillus subtilis S0507

ZHU Sai-feng, WU Jian-feng, ZHAO Xi-rong   

  1. 1. School of Chemical Engineering and Life Science, Huaiyin Institute of Technology, Huai’an 223003, China;
    2. Jiangsu King’s Luck Brewery Joint-Stock Co. Ltd., Lianshui 223411, China
  • Online:2014-05-15 Published:2014-05-19

摘要:

通过响应面分析法对枯草芽孢杆菌S0507产四甲基吡嗪(tetramethylpyrazine,TTMP)培养工艺进行优化。经单因素试验后,以Box-Behnken法设计考察培养时间、培养温度、水分含量3 个因素对TTMP产量的交互影响,用Design-Expert v8.0.1.6软件对BBD试验数据进行分析处理。通过响应面试验得到的最佳培养条件为:低温培养时间33.84 h、培养温度41.75 ℃、水分含量59.63%,TTMP产量为332.70 mg/kg,理论值(335.49 mg/kg)与实验值的相对偏差为0.84%,证明应用响应曲面法优化枯草芽孢杆菌S0507产TTMP的培养条件是可行的。

关键词: 四甲基吡嗪, 枯草芽孢杆菌, 响应面法, 气相色谱分析

Abstract:

Tetramethylpyrazine (TTMP) was prepared from Bacillus subtilis S0507 under solid-state fermentation. Response
surface methodology (RSM) was applied to optimize the culture conditions. On the basis of the results of one-factor-at-atime
experiments, a Box-Behnken design (BBD) was used to investigate the interactive effect of culture temperature, time
and solid-to-liquid ratio (water content of the solid medium) on TTMP production, and the experimental data obtained
were analyzed with Design-Expert v8.0.1.6 software. The optimal culture conditions as determined by response
surface analysis were 33.84 h, 41.75 ℃ and water content of 59.63%. The measured yield of TTMP under the optimized
conditions was 332.70 mg/kg, showing a relative deviation of 0.84% in comparison with the theoretically predicted
value of 335.49 mg/kg. Thus, response surface methodology is feasible to optimize the culture conditions for TTMP
production by Bacillus subtilis S0507.

Key words:  tetramethylpyrazine, Bacillus subtilis, response surface methodology, gas chromatography (GC)