食品科学

• 工艺技术 • 上一篇    下一篇

富含岩藻糖苹果皮果胶的提取与理化性质

程 洋,张 英,段双艳,黄琳娟,王仲孚*   

  1. 西北大学生命科学学院,西部资源生物与现代生物技术教育部重点实验室,陕西省生物技术重点实验室,陕西 西安 710069
  • 出版日期:2014-05-25 发布日期:2014-05-29
  • 通讯作者: 王仲孚
  • 基金资助:

    国家自然科学基金面上项目(31071506);陕西省教育厅自然科学基金项目(2013JK0714)

Extraction and Physico-chemical Properties of Fucose-Rich Pectins from Apple Peel

CHENG Yang, ZHANG Ying, DUAN Shuang-yan, HUANG Lin-juan, WANG Zhong-fu*   

  1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education,
    Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Science, Northwest University, Xi’an 710069, China
  • Online:2014-05-25 Published:2014-05-29
  • Contact: WANG Zhong-fu

摘要:

以超声辅助柠檬酸提取法从苹果皮中提取苹果果胶,并对所提取苹果皮果胶进行理化性质测定和分析。在最佳提取条件下(pH 2.5柠檬酸,450 W超声30 min),苹果皮果胶得率为14.6%。理化性质分析表明:所得苹果皮果胶的酯化度为69.9%、糖醛酸含量为58.5%、总糖含量为92.8%,分子质量大于400 kD。气相色谱法分析表明:该方法提取的苹果皮果胶由鼠李糖、岩藻糖、阿拉伯糖、木糖、葡萄糖、半乳糖、葡萄糖醛酸及半乳糖醛酸8 种单糖组成,单糖组成的物质的量比为9.5∶19.2∶4.6∶0.1∶4.6∶3.6∶2.3∶56.3,岩藻糖含量高达19.2%。而超声法、盐酸法提取的苹果皮果胶中未检测到岩藻糖。进一步研究表明:富含岩藻糖的苹果皮果胶的提取与柠檬酸作萃取剂有一定关系。

关键词: 苹果皮果胶, 提取, 岩藻糖, 理化性质

Abstract:

In this study, apple pectins (APs) were extracted from apple peel by ultrasound-assisted extraction with citric
acid, and investigated for physico-chemical properties. Under the optimized conditions of pH 2.5 citric acid solution as the
extraction solvent, 450 W ultrasound power and 30 min radiation time, the yield of APs was 14.6%. The extracted APs were
characterized by a degree of esterification of 69.9% and a molecular weight larger than 400 kD, and contained 92.8% total
sugar, 58.5% aldonic acid. GC data indicated that the APs consisted of eight monosaccharides including Rha, Fuc, Ara, Xyl,
Glc, Gal, GlcA and GalA, with a relative molar ratio of 9.5:19.2:4.7:0.1:4.6:3.6:2.3:56.3 and a significantly high percentage
of Fuc, reaching 19.2%. Further results revealed that the extraction of Fuc-rich APs was likely associated with the method
using citric acid as the extraction solvent.

Key words: apple pectin, extraction, fucose, physico-chemical properties, citric acid

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