食品科学

• 工艺技术 • 上一篇    下一篇

响应面法优化提高发酵桑叶茶中1-脱氧野尻霉素含量工艺

洪1,黄先智2,沈以红2,丁晓雯1,*,秦樱瑞1,曾艺涛1,杨 娟1,丁顺杰1,商 桑1   

  1. 1.西南大学食品科学学院,重庆市农产品加工重点实验室,重庆 400715;
    2.家蚕基因组生物学国家重点实验室,重庆 400715
  • 出版日期:2014-05-25 发布日期:2014-05-29
  • 通讯作者: 丁晓雯
  • 基金资助:

    国家现代农业(蚕业)产业技术体系建设专项(CARS-22-ZJ0503);重庆市科委应用技术项目(cstc,2012gg-yyjs80022)

Optimization of Fermentation Conditions for Enhanced 1-Deoxynojirimycin Content in Fermented Mulberry Leaf Tea by Response Surface Methodology

XIAO Hong1, HUANG Xian-zhi2, SHEN Yi-hong2, ,*, QIN Ying-rui1, ZENG Yi-tao1, YANG Juan1, DING Shun-jie1, SHANG Sang1   

  1. 1. Chongqing Key Laboratory of Agricultural Product Processing, College of Food Science, Southwest University,
    Chongqing 400715, China; 2. State Key Laboratory of Silkworm Genome Biology, Chongqing 400715, China
  • Online:2014-05-25 Published:2014-05-29
  • Contact: DING Xiao-wen

摘要:

为优化提高发酵桑叶茶中1-脱氧野尻霉素含量工艺,采用响应面分析法,以发酵温度、发酵时间及接种量为自变量,1-脱氧野尻霉素含量为响应值,设计三因素三水平响应面回归分析。结果表明:提高发酵桑叶茶中1-脱氧野尻霉素含量工艺的最佳工艺条件为发酵温度30 ℃、发酵时间5.6 h、黑曲霉∶日本根霉∶绿色木霉=2∶1∶2菌液接种量3.75×107 CFU/100 g,在此条件下发酵桑叶茶中1-脱氧野尻霉素含量为133.882 mg/100 g。

关键词: 发酵, 桑叶茶, 1-脱氧野尻霉素, 响应面分析

Abstract:

Response surface methodology was used to optimize the fermentation conditions for producing fermented
mulberry leaf tea with higher 1-deoxynojirimycin (1-DNJ) content. The 1-DNJ content was investigated with respect to
fermentation temperature, fermentation time and inoculum amount by response surface regression analysis. The optimum
fermentation conditions that provided a 1-DNJ content of 133.882 mg/100 g dry weight of fermented mulberry leaf tea were
determined as 30 ℃, 5.6 h, a ratio of Aspergillus niger to Rhizopus japonicus Vuillemin to Trichoderma viride of 2:1:2, and
an inoculum amount of 3.75×107 CFU/100 g.

Key words: fermentation, mulberry leaves, 1-deoxynojirimycin (1-DNJ), response surface analysis

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