关键词: 长白落叶松, 二氢槲皮素, 纤维素酶, 闪式提取, 响应面分析法

Abstract:

Objective: To optimize the celluase-assisted extraction of dihydroquercetin from Larix olgensis Henry var.
koreana Nakai wood chips by response surface methodology (RSM). Methods: Dihydroquercetin was extracted with ethanol
in a herbal blitzkrieg extractor from pine wood chips pre-hydrolyzed with celluase. On the basis of the results of single-factor
experiments, a Box-Behnken design was performed to optimize three extraction conditions including ethanol concentration,
enzyme dosage and liquid-to-solid ratio. Results: The optimal extraction conditions were determined as hydrolysis with an
enzyme dosage of 3.48 mg/g and subsequent extraction for 120 s with 60.93% ethanol in water at a liquid-to-solid ratio of
103.64:1 (mL/g). Conclusion: The proposed extraction procedure can provide a simple, energy-saving and time-saving way
for massive production of dihydroquercetin.

Key words: Larix olgensis Henry var. koreana Nakai, dihydroquercetin, cellulase, extraction, response surface methodology (RSM)