食品科学

• 基础研究 • 上一篇    下一篇

α-松油醇对意大利青霉的抑制作用

欧阳秋丽,贾 雷,陶能国*,何湘丽   

  1. 湘潭大学化工学院,湖南 湘潭 411105
  • 出版日期:2014-06-15 发布日期:2014-07-03

Inhibitory Effect of α-Terpineol on Penicillium italicum

OUYANG Qiu-li, JIA Lei, TAO Neng-guo*, HE Xiang-li   

  1. College of Chemical Engineering, Xiangtan University, Xiangtan 411105, China
  • Online:2014-06-15 Published:2014-07-03

摘要:

测定α-松油醇对意大利青霉菌丝体形态及菌丝体生长的影响,并对其抑菌机制进行初步探讨。结果表明:α-松油醇能明显抑制意大利青霉菌丝体生长,最小抑菌浓度(minimal inhibitory concentration,MIC)和最小杀菌浓度(minimal fungicidal concentration,MFC)分别为2.00 μL/mL和8.00 μL/mL;经MIC和MFC α-松油醇处理后,意大利青霉菌丝体胞外pH值、胞外电导率及260 nm条件下吸光度显著增加,总脂质含量有显著下降。提示α-松油醇能改变细胞膜的通透性、破坏细胞膜结构的完整性,导致胞内成分泄露,从而抑制意大利青霉菌丝体生长。

关键词: &alpha, -松油醇, 意大利青霉, 细胞膜, 抑制作用

Abstract:

The effect of α -terpineol at various concentrations on the morphology and mycelial growth of Penicillium
italicum was evaluated, and the corresponding mechanism was explored. Results showed that α-terpineol had a significant
inhibitory effect on the mycelial growth of P. italicum, with minimal inhibitory concentration (MIC) and minimal fungicidal
concentration (MFC) of 2.00 and 8.00 μL/mL, respectively. After being treated with α-terpineol at MIC or MFC, marked
increases in the extracellular pH, extracellular conductivity and absorbance at 260 nm were observed. Moreover, a decrease
in total lipid content was also observed. Our research indicated that α-terpineol can increase the membrane permeability
and destroy the integrity of the membrane of P. italicum, thus resulting in the leakage of cellular materials and inhibiting the
mycelial growth of P. italicum.

Key words: α-terpineol, Penicillium italicum, cell membrane, inhibitory effect