食品科学

• 生物工程 • 上一篇    下一篇

硫色镰刀菌(Fusarium sulphureum)体外产毒条件的筛选

唐亚梅,薛华丽,毕 阳*,赵 莹,沈科萍,王 毅   

  1. 甘肃农业大学食品科学与工程学院,甘肃 兰州 730070
  • 发布日期:2014-07-03

Screening of Conditions for Mycotoxin Production by Fusarium sulphureum in vitro

TANG Ya-mei, XUE Hua-li, BI Yang*, ZHAO Ying, SHEN Ke-ping, WANG Yi   

  1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China
  • Published:2014-07-03

摘要:

对影响硫色镰刀菌(Fusarium sulphureum)体外产生真菌粗毒素的温度、时间、pH值、培养方式及培养基种类进行筛选。结果表明:F. sulphureum的最佳产毒条件是Richard培养基、25 ℃、pH 4.5、振荡培养10 d。在单因素筛选的基础上,以温度、pH值和培养时间为自变量,绿豆种子胚根平均长度为响应值,利用响应面分析法对该菌产毒条件的优化结果表明,22.2 ℃、pH 5.1、振荡培养12 d最有利于体外产毒。

关键词: 硫色镰刀菌, 粗毒素, 响应面法, 产毒条件

Abstract:

Fusarium sulphureum is a major pathogen causing dry rot of potato tubers, and has a strong ability to produce
mycotoxins. The incubation conditions for this pathogenic strain to produce mycotoxin in vitro were screened including
temperature, time, pH and stirring condition (static or shaking) and medium type. The results showed that the optimum
conditions for mycotoxin production were shaking culture at 25 ℃ for 10 days in Richard liquid medium at pH 4.5.
Temperature, pH and culture time were selected by using one-factor-at-a-time designs for further optimization by response
surface analysis using the average length of mung bean seed radicles as the response variable. A culture temperature of
22.2 ℃, pH 5.1 and 12 d culture were found optimal for mycotoxin production in vitro.

Key words: Fusarium sulphureum, crude toxin, response surface methodology, toxin production condition