食品科学

• 生物工程 • 上一篇    下一篇

响应面法优化豆乳链球菌增殖培养基

刘丽莎,陶国琴,郭 宏,吕晓莲,贾建会,彭义交   

  1. 1.北京市食品研究所,北京 100162;2.北京食品科学研究院,北京 100068;
    3.北京二商集团有限责任公司,北京 100053
  • 出版日期:2014-06-15 发布日期:2014-07-03

Optimization of Culture Medium for Streptococcus sojalactis SY1.1 Using Response Surface Methodology

LIU Li-sha, TAO Guo-qin, GUO Hong, LÜ Xiao-lian, JIA Jian-hui, PENG Yi-jiao   

  1. 1. Beijing Food Research Institute, Beijing 100162, China; 2. Beijing Academy of Food Sciences, Beijing 100068, China;
    3. Beijing Er Shang Group Co. Ltd., Beijing 100053, China
  • Online:2014-06-15 Published:2014-07-03

摘要:

针对优良酸豆乳发酵菌株豆乳链球菌(Streptococcus sojalactis)SY1.1增殖培养基进行优化,研究碳氮源对豆乳链球菌生长的影响,并采用响应面法优化豆乳链球菌SY1.1的增殖培养基配方。结果表明:以AST为基础培养基,优选大豆蛋白胨和蔗糖为最适氮源和碳源,质量比为1∶2,采用Plackett-Burman设计对11 种促乳酸菌生长因子进行评价,利用中心旋转组合设计和响应面分析确定增殖培养基的配方为:蔗糖10 g/L、大豆蛋白胨20 g/L、磷酸氢二钾2 g/L、番茄汁85.8 mL/L、胡萝卜汁109 mL/L、玉米浆6 g/L,pH 6.8。SY1.1以接种量2%,在37 ℃培养12 h时,活菌数可达(8.9±0.2)×108 CFU/mL,较初始豆浆培养基的活菌数(1.03×108 CFU/mL)提高了7.64 倍。

关键词: 豆乳链球菌, 增殖培养基, Plackett-Burman设计, 响应面法优化

Abstract:

In this paper, response surface methodology was used to optimize the composition of AST-based culture medium
for Streptococcus sojalactis SY1.1 to obtain high density of bacteria. Through single-factor experiments, the optimum carbon
source and nitrogen source were found to be sucrose and soy peptone, respectively. The major medium components for
SY1.1 were screened by Plackett-Burman design. Then, the optimum culture medium was determined to consist of 10 g/L
sucrose, 20 g/L soy peptone, 2 g/L dipotassium phosphate, 85.8 mL/L tomato juice, 109 mL/L carrot juice and 6 g/L corn
steep liquor at pH 6.8 by central composite design and response surface analysis. The cell production of SY1.1 cultured in the
optimized medium for 12 h at 37 ℃ was (8.9±0.2) ×108 CFU/mL, which was 8.64 times higher than that (1.03×108 CFU/mL)
observed with soymilk as initial medium.

Key words: Streptococcus sojalactis, optimum culture medium, Plackett-Burman, response surface optimization