食品科学

• 基础研究 • 上一篇    下一篇

蜂胶中多酚类成分分析及其抗氧化活性

张红城,赵亮亮,胡 浩,董 捷   

  1. 中国农业科学院蜜蜂研究所,国家农产品加工中心蜂产品加工分中心,北京 100093
  • 出版日期:2014-07-15 发布日期:2014-07-18

Polyphenolic Composition and Antioxidant Properties of Propolis

ZHANG Hong-cheng, ZHAO Liang-liang, HU Hao, DONG Jie   

  1. National R&D Centre for Bee Product Processing, Bee Research Institute, Chinese Academy of Agricultural Sciences,Beijing 100093, China
  • Online:2014-07-15 Published:2014-07-18

摘要:

为研究不同地区蜂胶提取物中组分含量的差异及抗氧化活性的大小,采用75%乙醇溶液对9 个蜂胶样品进行提取,以高效液相色谱法(high performance liquid chromatography,HPLC)对不同提取物中多酚类成分进行定性和定量分析。并利用氧化自由基吸收能力(oxygen radical absorbance capacity,ORAC)法和用流式细胞仪Hep G2细胞模型评估了9 个蜂胶样品的体外和细胞水平抗氧化能力。结果表明:蜂胶样品中含有包括黄酮、酚酸及酯类物质在内的大量多酚类成分,其中含量较高的黄酮类成分有短叶松素-3-乙酸酯、松属素、柯因、短叶松素、高良姜素,含量较高的酚酸及酯类成分有咖啡酸苯乙酯、p-香豆酸、咖啡酸、异阿魏酸。ORAC法结果显示所有蜂胶样品都具有很好的体外抗氧化能力;在细胞水平上,所有样品也都表现出一定的抗氧化能力。9 个总黄酮和总酚酸含量高的蜂胶具有较强的抗氧化能力。

关键词: 蜂胶, 多酚类化合物, 抗氧化

Abstract:

In order to evaluate the polyphenolic composition and antioxidant activity of propolis collected from different
areas, nine propolis samples were extracted with 75% aqueous ethanol. The polyphenolic composition of the resulting
extracts was analyzed by high performance liquid chromatography-diode array detector (HPLC-DAD). Furthermore, the
antioxidant capacity of the extracts was evaluated in vitro by oxygen radical absorbance capacity (ORAC) as well as Hep G2
cellular assays by flow cytometry. All the propolis samples were rich in polyphenolics, including flavonoids, phenolic acids
and esters. The flavonoids mainly included pinobanksin-3-O-acetate, pinocembrin, chrysin, pinobanksin and galangin; the
phenolic acids and related esters mainly included caffeic acid phenethyl ester, p-coumaric acid, caffeic acid and isoferulic
acid. In addition, the ORAC assay showed that all the propolis samples had free-radical scavenging activities; the same
results were obtained in Hep G2 cellular assays. Our results indicated that propolis with strong antioxidant capacity usually
contained large amounts of polyphenolic compounds.

Key words: propolis, polyphenolics, antioxidant properties