关键词: 长裙竹荪, 多糖, 糖谱法, 荧光辅助凝胶电泳, 多角度激光光散射

Abstract:

Water soluble polysaccharides from fruiting body of Dictyophora indusiata (D. indusiata) collected from different
places of China were efficiently extracted by using microwave-assisted extraction. The molecular parameters and chemical
characters were investigated and compared using high performance size exclusion chromatography coupled with multiangle
laser light scattering (HPSEC-MALLS), and saccharide mapping based on polysaccharide analysis by carbohydrate gel
electrophoresis (PACE), respectively. The results showed that the HPSEC chromatograms, the molecular weights and their
distribution of polysaccharides from different samples of D. indusiata were similar. All α-1,4(6)- and β-1,3(4)-glucosidic,
β-2,1-fructosidic and α-1,4-galactouronic linkages existed in polysaccharides from D. indusiata. Their similarity of partial
acid and enzymatic hydrolysates of polysaccharides in D. indusiata collected from different regions was high. The results are
helpful to better understand the polysaccharides from D. indusiata, and beneficial to improve their quality control.

Key words: Dictyophora indusiata, polysaccharides, saccharide mapping, polysaccharide analysis by carbohydrate gel electrophoresis (PACE), multi-angle laser light scattering (MALLS)