乳源酪蛋白糖巨肽对结肠癌HT-29细胞中COX-2、iNOS、GST-π表达的影响

doi:10.7506/spkx1002-6630-201413041

食品科学

乳源酪蛋白糖巨肽对结肠癌HT-29细胞中COX-2、iNOS、GST-π表达的影响

曹江鸣，陈庆森，阎亚丽，庞广昌

天津市食品生物技术重点实验室，天津商业大学生物技术与食品科学学院，天津 300134

出版日期:2014-07-15 发布日期:2014-07-18

Effect of Casein Glycomacropeptide (CGMP) on COX-2, iNOS and GST-π Expression in HT-29 Cells

CAO Jiang-ming, CHEN Qing-sen, YAN Ya-li, PANG Guang-chang

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce,
Tianjin 300134, China

Online:2014-07-15 Published:2014-07-18

摘要/Abstract

摘要：

目的：研究乳源酪蛋白糖巨肽（casein glycomacropeptide，CGMP）对人结肠癌HT-29细胞增殖及细胞中环氧合酶-2（cyclooxyenase-2，COX-2）、诱导性一氧化氮合酶（inducible nitric oxide synthase，iNOS）及谷胱甘肽-S-转移酶π（glutathione-S-transferase π，GST-π）表达的影响，为探讨CGMP作为功能性食品提供可靠的依据。方法：采用噻唑蓝（methyl thiazolyl tetrazolium，MTT）实验测定不同剂量CGMP作用12、24、48 h对结肠癌HT-29细胞增殖的抑制情况；在不同剂量CGMP作用HT-29细胞24 h后，通过逆转录聚合酶链反应扩增细胞中提取的COX-2 mRNA、iNOS mRNA、GST-π mRNA，用凝胶成像自动分析系统检测各扩增带COX-2 mRNA、iNOS mRNA、GST-π mRNA水平。结果：1）CGMP组对细胞的增殖均有抑制作用，其中10-4 mg/mL抑制效果最强，且呈现时间依赖性。2）低剂量的CGMP（10-5、10-4、10-3 mg/mL）可显著降低3 种基因的表达。结论：CGMP可在一定程度上抑制HT-29细胞的增殖，并呈时间依赖性，同时 CGMP能在mRNA水平上抑制COX-2、iNOS、GST-π的表达，进而降低3 种基因蛋白的表达，从而进一步改善结直肠癌。

关键词: 乳源酪蛋白糖巨肽, HT-29细胞, COX-2, iNOS, GST-&pi

Abstract:

Objective: The effect of casein glycomacropeptide (CGMP) on cell proliferation and on the expression of
cyclooxyenase-2 (COX-2), inducible nitrite oxide synthase (iNOS), and glutathione-S-transferase π (GST-π) in HT-29
cells was explored to provide a reliable basis for the use of CGMP as an ingredient in functional foods. Methods: HT-29
Cells were cultured with CGMP for 12, 24 and 48 h. The inhibition of HT-29 cells proliferation was measured by MTT
[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The expression of COX-2, iNOS, and GST-π was
detected by reverse transcriptionpolymerase chain reaction (RT-PCR) after the HT-29 cells were treated for 24 h. Results: 1)
CGMP inhibited the proliferation of HT-29 cells in a time-dependent fashion, and the optimal concentration was 10-4 mg/mL.
2) Low doses of CGMP (10-5, 10-4 and 10-3 mg/mL) significantly reduced the expression of three genes. Conclusion: To some
extent, CGMP can inhibit the proliferation of HT-29 cells in a time-dependent manner. The mechanism is associated with
decreasing the expression of COX-2, iNOS and GST-π by CGMP and further improving human colorectal cancer.

Key words: casein glycomacropeptide (CGMP), human colonic tumor cells HT-29, COX-2, iNOS, GST-π

曹江鸣，陈庆森，阎亚丽，庞广昌. 乳源酪蛋白糖巨肽对结肠癌HT-29细胞中COX-2、iNOS、GST-π表达的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201413041.

CAO Jiang-ming, CHEN Qing-sen, YAN Ya-li, PANG Guang-chang. Effect of Casein Glycomacropeptide (CGMP) on COX-2, iNOS and GST-π Expression in HT-29 Cells[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201413041.

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乳源酪蛋白糖巨肽对结肠癌HT-29细胞中COX-2、iNOS、GST-π表达的影响

Effect of Casein Glycomacropeptide (CGMP) on COX-2, iNOS and GST-π Expression in HT-29 Cells

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