食品科学

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下关生沱茶的成分分析以及对SD大鼠的盐酸/乙醇诱导胃损伤预防效果

王 睿,赵 欣   

  1. 重庆第二师范学院生物与化学工程系,重庆 400067
  • 出版日期:2014-07-15 发布日期:2014-07-18

Chemical Composition of Xiaguan Raw Tuocha and Its Preventive Activity on HCl/Ethanol-Induced Gastric Injury in SD Rats

WANG Rui, ZHAO Xin   

  1. Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China
  • Online:2014-07-15 Published:2014-07-18

摘要:

为弄清下关生沱茶(Xiaguan raw Tuocha,XRT)的成分和其体内胃损伤预防效果,本实验利用LC-MS和细胞因子分析,同时还测定了胃液量和胃液的pH值。结果表明:XRT具有12 种成分,这些成分使XRT具有胃损伤预防效果。高质量浓度的XRT相比低质量浓度的XRT,细胞因子IL-6 和TNF-α下降。1 000 mg/kg的XRT处理时小鼠显示出和正常小鼠接近的低胃液量(2.0 mL)和高的pH值(3.1)。由此可知,下关生沱茶包含很多功能性成分并且表现出强有力的胃损伤预防效果。

关键词: 沱茶, 胃损伤, 细胞因子, 大鼠

Abstract:

Xiaguan Tuocha is a functional drink and known to possess some health properties. In order to identify the
chemical components and investigate preventive effects of Xiaguan raw (unfermented) Tuocha (XRT) on gastric injury
in vivo, liquid chromatography-mass spectrometry (LC-MS) and cytokine analysis were used in this study, and the gastric
secretion and pH of gastric juice were also tested. Twelve components were found in XRT, all of which contributed to the
preventive effect on gastric injury. XRT at higher concentration reduced the levels of serum proinflammatory cytokines such
as IL-6 and TNF-α compared with at lower concentration. The 1 000 mg/kg XRT showed the best inhibitory effect on gastric
injury (78.7% inhibitory rate). The 1 000 mg/kg XRT treated rats also exhibited similarly low gastric secretion (2.0 mL) and
higher pH of gastric juice (pH 3.1) compared with normal rats. Xiaguan Tuocha contained many functional components and
exerted a strong preventive effect on gastric injury.

Key words: Tuocha, gastric injury, cytokine, rat