蓝莓果汁及不同纯度蓝莓花色苷对SHR血压的影响

摘要/Abstract

摘要： 摘要：以蓝莓果汁及不同纯度蓝莓花色苷提取物为原料灌胃12周龄原发性高血压大鼠(SHR)和 Wistar 大鼠，研究花色苷提取纯化对其降压效果影响。采用蓝莓果汁（A）（花色苷的质量分数为1.00%）及不同纯度的花色苷（花色苷含量的质量分数分别为8.66%(B)、38.49%(C)、94.42%(D)）灌胃原发性高血压大鼠（SHR），灌胃剂量以花色苷计。其中，A的灌胃剂量为94.4、472.1、944.2 mg/kg m，B的灌胃剂量为10.9、54.5、109.0 mg/kg m，C的灌胃剂量为2.5、12.3、24.5 mg/kg m，D的灌胃剂量为1.0、5.0、10.0 mg/kg m。结果表明：灌胃同剂量（折合花青素）的蓝莓果汁与蓝莓皮渣提取物（不同纯度花色苷提取物)相比，其对SHR的降压效果有显著差异（p＜0.05），而同剂量的蓝莓皮渣提取物对SHR 的降压效果无显著差异(p＞0.05)。其中，高剂量果汁(A)和不同纯度花色苷（B,C,D）灌胃SHR，灌胃后在4或5小时其收缩压分别降低了38.45、32.57、33.09 、33.41mmHg。因此，蓝莓对高血压改善作用的功能成分是花色苷；蓝莓果汁的其他成分对花色苷的降血压功能有增强作用。

关键词: 关键字：蓝莓果汁, 花色苷, 高血压, 原发性高血压大鼠

Abstract: Abstract：In order to investigate effect of blueberry juice and different purity blueberry anthocyanins on blood pressure of spontaneously hypertensive rat(SHR), blueberry juice (A) (mass fraction of anthocyanins is 1.00% ) and different purity anthocyanins (mass fraction of anthocyanins is 8.66% (B) , 38.49% (C), 94.42% (D)) were prepared, and then were to oral gavaged 12-week-old male SHR and Wistar rats with different doses (A: 94.4, 472.1, 944.2 mg/kg m; B: 10.9, 54.5, 109.0 mg/kg m; C: 2.5, 12.3, 24.5 mg/kg m; D: 1.0, 5.0, 10.0 mg/kg m). The results showed that it has significant difference （p＜0.05）using blue berry juice and extractive from blueberry skin slag to oral gavage SHR in the same dose( same quality in anthocyanin), and it has not significant difference （p＞0.05）using extractive from blueberry skin slag to oral gavage SHR in the same dose( same quality in anthocyanin). Using blue berry juice(A) and extractive from blueberry skin slag(B, C and D )to oral gavage SHR in high dose( same quality in anthocyanin), after 4h or 5h, SHR systolic pressure gone down 38.45、32.57、33.09 、33.41mmHg, respectively. Therefore，the functional ingredients to hypertension improvement in blueberry are anthocyanins; other ingredients of blueberry juice can enhance the antihypertensive function of anthocyanins.

Key words: Key word: Blueberry juice, anthocyanins, hypertension, spontaneously hypertensive rats (SHR)

中图分类号:

中图分类号：TS255.44

马立志李金星刘志刚周笑犁胡志和. 蓝莓果汁及不同纯度蓝莓花色苷对SHR血压的影响[J]. 食品科学, 0, (): 0-0.

Li-Zhi MA Zhi-He HU. Effect of Blueberry Juice and Different Purity Blueberry Anthocyanins on Blood Pressure of SHR[J]. FOOD SCIENCE, 0, (): 0-0.

参考文献

[1]张成喜, 潘思年, 蒙荣森, 等.二甲双胍对压力超负荷大鼠心肌肥厚的影响[J].中国病理生理杂志, 2010, 26(2):238-244 [2] Longo M B, Mulanda M J M, Nkiama E, et al.Effects of soccer/football practice on cardiovascular risk factors among young adults[J].Mali Medical, 2008, 23(4):23-28 [3]Gokce N.L-arginine and hypertension[J].The Journal of nutrition, 2004, 134(10):2807-S [4] Sessa W C.Regulation of endothelial derived nitric oxide in health and disease[J].Memórias do Instituto Oswaldo Cruz, 2005, 100(1):15-18 [5]Chrysohoou C, Panagiotakos D B, Pitsavos C, et al.The association between pre-hypertension status and oxidative stress markers related to atherosclerotic disease: the ATTICA study[J].Atherosclerosis, 2007, 192(1):169-176 [6]Pitsavos C, Chrysohoou C, Panagiotakos D B, et al.Abdominal obesity and inflammation predicts hypertension among prehypertensive men and women: the ATTICA Study[J].Heart and vessels, 2008, 23(2):96-103 [7]张茂云，牟宗毅 .非药物疗法治疗老年性高血压的研究进展[J].中国老年学杂志, 2013, 33(2):461-464 [8]Huang W Y, Davidge S T, Wu J.Bioactive Natural Constituents from Food Sources—Potential Use in Hypertension Prevention and Treatment[J].Critical reviews in food science and nutrition, 2013, 53(6):615-630 [9]胡志和, 张然嬉, 冯永强, 等.提取紫薯淀粉的副产物对血压的影响[J].食品科学, 2010, 31(21):361-364 [10]王佳佳, 胡志和, 蒋毅.富含抑制肽的酪蛋白水解物及其功能性酸奶对血压的影响[J].食品工业科技, 2013, 34(10):345-351 [11]杨智承，刘苑，谭毓治.抗高血压中药活性成分及作用机制研究进展[J].广东药学院学报, 2010, 26(1):102-105 [12] Lewis B J, Herrlinger K A, Craig T A, et al.Antihypertensive effect of passion fruit peel extract and its major bioactive components following acute supplementation in spontaneously hypertensive rats[J].The Journal of nutritional biochemistry, 2013, 24(7):1359-1366 [13] Hopkins A L, Lamm M G, Funk J L, et al.Hibiscus sabdariffa L. in the treatment of hypertension and hyperlipidemia: A comprehensive review of animal and human studies[J].Fitoterapia, 2013, 85(1):84-94 [14]Hassellund S S, Flaa A, Sandvik L, et al.Effects of anthocyanins on blood pressure and stress reactivity: a double-blind randomized placebo-controlled crossover study[J].Journal of human hypertension, 2012, 26(6):396-404 [15]Belmokhtar M, Bouanani N E, Ziyyat A, et al.Antihypertensive and endothelium-dependent vasodilator effects of aqueous extract of Cistus ladaniferus[J].Biochemical and biophysical research communications, 2009, 389(1):145-149 [16] Guerrero M F, Carron R, Martin M L, et al.Antihypertensive and vasorelaxant effects of aqueous extract from Croton schiedeanus Schlecht in rats[J].Journal of Ethnopharmacology, 2001, 75(1):33-36 [17]Luo Lifang, Wu Weihua, Zhou Yingjun, et al.Antihypertensive effect of Eucommia ulmoides Olivextracts in spontaneously hypertensive rats[J].Journal of ethnopharmacology, 2010, 129(2):238-243 [18]Cai Runlan, Li Meng, Xie Suhua, et al.Antihypertensive effect of total flavone extracts from Puerariae Radix[J].Journal of ethnopharmacology, 2011, 133(1):177-183 [19]史海芝, 刘惠民.国内外蓝莓研究现状[J].江苏林业科技, 2009, 36(4):48-51 [20]李颖畅, 孟宪军, 孙靖靖, 等.蓝莓花色苷的降血脂和抗氧化作用[J].食品与发酵工业, 2008, 34(10):44-48 [21]王秋霜, 凌彩金, 刘淑媚, 等.花青素分离纯化及其组分鉴定研究进展[J].食品与工业科技, 2013, 34(3):358-360 [22]Borges G, Degeneve A, Mullen W, et al.Identification of Flavonoid and Phenolic Antioxidants in Black Currants,Blueberries,Raspberries,Red Currants,and Cranberries?[J].Journal of agricultural and food chemistry, 2010, 58(7):3901-3909 [23]Lee J, Finn C E.Lingonberry (Vaccinium vitis-idaea L) grown in the Pacific Northwest of North America: Anthocyanin and free amino acid composition[J].Journal of Functional Foods, 2012, 4(1):213-218 [24]冷吉燕, 张婧, 邵明柏.蓝莓花色苷的研究进展[J].中国老年学杂志, 2011, 31(17):3419-3423 [25]Pasko P, Barton H, Zagrodzki P, et al.Anthocyanins,total polyphenols and antioxidant activity in amaranth and quinoa seeds and sprouts during their growth[J].Food Chemistry, 2009, 115(3):994-998 [26]Grace M H, Ribnicky D M, Kuhn P, et al.Hypoglycemic activity of a novel anthocyanin rich formulation from lowbush blueberry[J].Vaccinium Angustifolium Aiton Phytomedicine, 2009, 16(5):406-415 [27]Bakowska A, Kucharska A Z, Oszmianski J.The effects of heating,UV irradiation,and storage on stability of the anthocyanin-polyphenol copigment complex[J].Food Chemistry, 2003, 81(3):349-355 [28] 孟宪军, 于欣灵, 孙仁艳, 等.蓝莓提取物对小鼠记忆力及抗衰老作用的研究[J].沈阳农业大学学报, 2011, 42(6):740-742 [29]Neto C C, Amoroso J W, Liberty A M.Anticancer activities of cranberry phytochemicals: an update[J].Molecular Nutrition and Food Research, 2008, 52(1):S18-S27 [30]张燕, 王红英, 胡艳君, 等.大鼠血压尾套法测定影响因素探讨[J].西北药学杂志, 2008, 23(2):34-42 [31]刘军伟, 胡志和, 张祎豪, 等.紫薯水溶性提取物以及紫薯酸奶对血压的影响[J].食品工业科技, 2013, 34(8):353-357