乌龙茶多糖的聚酰胺柱层析法纯化工艺

doi:10.7506/spkx1002-6630-201414001

食品科学

乌龙茶多糖的聚酰胺柱层析法纯化工艺

张芸1,2，倪德江3，余志3，谢笔钧1,*

1.华中农业大学食品科技学院，湖北武汉 430070；2.湖北经济学院旅游与酒店管理学院，湖北武汉 430205；
3.华中农业大学园艺林学学院，园艺植物生物学教育部重点实验室，湖北武汉 430070

出版日期:2014-07-25 发布日期:2014-08-26
通讯作者: 谢笔钧
基金资助:
国家自然科学基金青年科学基金项目（31000316）

Purification of Oolong Tea Polysaccharides by Polyamide Column Chromatography

ZHANG Yun1,2, NI De-jiang3, YU Zhi3, XIE Bi-jun1,*

1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. College of Tourism
and Hotel Management, Hubei University of Economics, Wuhan 430205, China; 3. Key Laboratory of Horticultural Plant Biology,
Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China

Online:2014-07-25 Published:2014-08-26
Contact: XIE Bi-jun

摘要/Abstract

摘要：

为研究乌龙茶多糖（oolong tea polysaccharides，OTPS）聚酰胺柱层析脱色脱蛋白的效果，首先采用单因素试验，以脱色率、脱蛋白率和多糖保留率为评价指标，从上样量、上样体积、吸附平衡时间以及洗脱速率4 个方面确定了聚酰胺柱层析的洗脱条件。然后通过脱色率、脱蛋白率、多糖保留率、产品得率和抗氧化活性5 个方面，比较了OTPS聚酰胺柱层析与常用的Sevag-H2O2联用法脱色脱蛋白的效果。结果表明：OTPS聚酰胺柱层析法脱色脱蛋白的最佳工艺条件为4 mg OTPS/mL聚酰胺，2/5 倍柱体积去离子水溶解上样，吸附平衡20 min，3 mL/min速率洗脱；聚酰胺柱层析对OTPS的脱色率和脱蛋白率略低于Sevag-H2O2联用法，但多糖保留率、产品得率和抗氧化活性显著高于Sevag-H2O2联用法，而且方法简单，无环境污染，是一种较好的OTPS脱色脱蛋白方法，也可应用于其他多糖的脱色脱蛋白。

关键词: 乌龙茶多糖, 脱色, 脱蛋白, 聚酰胺柱层析

Abstract:

The purpose of this research was to estimate the effect of polyamide column chromatography on deproteinization
and decoloration of oolong tea polysaccharides (OTPS). The influences of sample concentration, sample loading volume,
adsorption equilibrium time as well as elution velocity on the decoloration rate, deproteinization rate and polysaccharide
retention rate of OTPS in polyamide column chromatography were studied. The polyamide column chromatography and
the traditional Sevag-hydrogen peroxide method used for deproteinization and decoloration were compared by decoloration
rate, deproteinization rate, polysaccharide retention rate, antioxidant activity and OTPS yield. The results showed that the
optimal conditions of polyamide column chromatography were as follows: sample loading at a level of 4 mg of OTPS
per mL polyamide column with a volume ratio of 2:5, followed by elution at a flow rate of 3 mL/min after 20 min of
adsorption equilibration. The decoloration rate and deproteinization rate of OTPS by polyamide column chromatography
were lower than those of the traditional method by Sevag-hydrogen peroxide, while significantly higher polysaccharide
retention rate, antioxidant activity and OTPS yield were obtained by polyamide column chromatography. The polyamide
column chromatography is a simple and environment-friendly method for deproteinization and decoloration of oolong tea
polysaccharides.

Key words: oolong tea polysaccharides, decoloration, deproteinization, polyamide column chromatography

中图分类号:

TS201.2

张芸1,2，倪德江3，余志3，谢笔钧1,*. 乌龙茶多糖的聚酰胺柱层析法纯化工艺[J]. 食品科学, doi: 10.7506/spkx1002-6630-201414001.

ZHANG Yun1,2, NI De-jiang3, YU Zhi3, XIE Bi-jun1,*. Purification of Oolong Tea Polysaccharides by Polyamide Column Chromatography[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201414001.

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乌龙茶多糖的聚酰胺柱层析法纯化工艺

Purification of Oolong Tea Polysaccharides by Polyamide Column Chromatography

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