食品科学

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自发气调对樱桃番茄果实蛋白表达变化的影响

周 翔,姜 丽,宦 晨,安秀娟,郁志芳*   

  1. 南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2014-07-25 发布日期:2014-08-26
  • 通讯作者: 郁志芳
  • 基金资助:

    公益性行业(农业)科研专项(2014030232)

Proteomic Analysis of Cherry Tomato Fruits under Modified Atmosphere Conditions

ZHOU Xiang, JIANG Li, HUAN Chen, AN Xiu-juan, YU Zhi-fang*   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095
  • Online:2014-07-25 Published:2014-08-26
  • Contact: YU Zhi-fang

摘要:

采用蛋白质组学方法研究自发气调(modified atmosphere,MA)对樱桃番茄果实保鲜的机理。经过双
向电泳和基质辅助激光解吸电离飞行时间质谱析,鉴定出39 个差异蛋白(P<0.05),根据功能分为6 类:胁迫
与防御蛋白(30.77%)、能量蛋白(25.64%)、细胞命运蛋白(15.38%)、成熟衰老蛋白(5.13%)、解毒蛋白
(5.13%)和未分类蛋白(17.95%);蛋白功能分析的结果显示,MA增强了谷胱甘肽转移酶、NADP-苹果酸酶、
真核转录起始因子5A-4和乙醇脱氢酶2的表达,抑制了水杨酸绑定蛋白2和1-氨基环丙烷-1-羧酸氧化酶的表达,表明
MA能通过减缓乙烯合成速率以及提高樱桃番茄果实的抗胁迫能力,从而延缓成熟衰老。

关键词: 自发气调, 樱桃番茄, 蛋白质组学, 延缓成熟衰老

Abstract:

Proteomic analysis was used to understand the response mechanism of cherry tomato fruits during storage
under modified atmosphere (MA) conditions in this experiment. After two-dimensional electrophoresis and matrix-assisted
laser desorption-ionization time of flight mass spectrometry (MALDI-TOF-TOF-MS), the results showed that there were
39 differently expressed spots (P < 0.05), including 6 functional proteins, stress response and defense (30.77%), energy
(25.64%), cell fate (15.38%), ripening and senescence (5.13%), detoxification (5.13%) and others (17.95%). Among these
proteins, glutathione S-transferase (GST), NADP-malic enzyme, eukaryotic translation initiation factor 5A-4 (eIF5A-4) and
alcohol dehydrogenase 2(ADH 2) were increased by MA treatment, while salicylic acid-binding protein 2 (SABP 2) and
1-aminocyclopropane-1-carboxylate oxidases (ACOs) were suppressed. This study indicated that the MA treatment of postharvest
cherry tomato fruits could induce the stress response and suppress the ethylene metabolism to delay the ripening of
cherry tomato fruits.

Key words: cherry tomato fruit, modified atmosphere, proteomics, delaying ripening and senescence

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