食品科学

• 基础研究 • 上一篇    下一篇

富硒小麦提取物中硒含量及其抗氧化特性

赵 萍,刘笑笑,王 雅,张 轶,郭 涛   

  1. 兰州理工大学生命科学与工程学院,甘肃 兰州 730050
  • 出版日期:2014-08-15 发布日期:2014-08-25

Selenium Content and Antioxidant Activity of Selenium-Enriched Wheat

ZHAO Ping, LIU Xiao-xiao, WANG Ya, ZHANG Yi, GUO Tao   

  1. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China
  • Online:2014-08-15 Published:2014-08-25

摘要:

为研究富硒小麦的富硒水平和硒的赋存形态,选用兰州高硒地区生产的富硒小麦为原料,通过超声、离心、透析的方法将富硒小麦中的有机硒和无机硒分离,用火焰原子吸收光谱法测定小麦硒水平。结果表明,富硒小麦硒的主要赋存形态是有机态,占总硒的84.36%。有机态硒主要为硒蛋白(54.46%),硒多糖次之,少量为硒核酸和其他有机态硒。在蛋白态硒中,清蛋白和谷蛋白硒含量较高,分别占总硒的19.46%和16.83%。抗氧化性分析结果表明,多糖相对于蛋白和核酸提取物,具有更长的诱导时间、更高的1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力和Fe3+还原力。皮尔逊相关性分析表明,小麦富硒提取物抗氧化能力与其硒含量之间表现为显著的正相关性。

关键词: 富硒小麦, 赋存形态, 硒含量, 火焰原子吸收光谱法, 抗氧化能力

Abstract:

In order to investigate selenium (Se) levels and speciation in Se-enriched wheat, organic and inorganic selenium
(Se) from wheat samples grown in Lanzhou were separated by ultrasonic pretreatment, centrifugation and dialysis and
determined by flame atomic absorption spectrometry. The results showed that Se existed mainly in organic form, accounting
for 84.36% of the total Se in Se-enriched wheat. The organic Se was mainly bound to protein (54.46%), followed by
polysaccharide, and a small amount of Se was bound to nucleic acid or other organic components. Albumin and glutelin
comprised 19.46% and 16.83% of total Se-containing proteins, respectively. The results of antioxidant activity showed
that the polysaccharides had longer induction time and higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging
activity and reducing activity than protein extracts and nucleic acid extracts. Pearson correlation analysis indicated that the
antioxidant capacity of Se-enriched wheat extracts had a significant positive correlation with Se contents.

Key words: selenium-enriched wheat, speciation, selenium content, flame atomic absorption spectrometry, antioxidant capacity

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