食品科学

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内生真菌拟茎点霉B3产漆酶分批发酵动力学

周 骏,梅艳珍,杨 腾,康 莉,戴志东,戴传超   

  1. 1.南京师范大学生命科学学院,江苏省微生物与功能基因组学重点实验室,江苏省微生物资源产业化工程技术研究中心,
    江苏 南京 210023;2.尚德生物科技有限公司,江苏 丹阳 212300
  • 出版日期:2014-08-15 发布日期:2014-08-25

Kinetics of Laccase Production by Batch Fermentation of Endophytic Fungus Phomopsis liquidambari B3

ZHOU Jun, MEI Yan-zhen, YANG Teng, KANG Li, DAI Zhi-dong, DAI Chuan-chao   

  1. 1. Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for
    Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China;
    2. Shangde Biotechnology Company, Danyang 212300, China
  • Online:2014-08-15 Published:2014-08-25

摘要:

通过建立内生真菌拟茎点霉(Phomopsis liquidambari)B3产漆酶分批发酵动力学模型,发现B3菌漆酶生成与菌体生长呈现部分生长偶联型。应用MATLAB软件将实验数据与符合B3菌菌体生长的Logistic模型、漆酶生成的Luedeking-Piret模型和基质消耗的Luedeking-Piret-Like模型进行非线性拟合,求得最优参数估计值和发酵动力学模型。分析动力学模型的拟合曲线,发现模型的曲线与实验值能较好地拟合。验证实验表明模型计算值与实验值的偏差大部分都低于10%,说明该动力学模型能很好地描述B3菌的实际发酵情况。

关键词: 内生真菌, 拟茎点霉, 漆酶, 分批发酵, 动力学模型

Abstract:

The aim of this study was to establish a kinetic model of laccase production by batch fermentation of the
endophytic fungus Phomopsis liquidambari B3. The experimental results showed that the production of laccase was partially
associated with the growth of P. liquidambari B3. The experimental data were nonlinearly fitted to the Logistic equation
describing cell growth, the Luedeking-Piret equation indicating laccase production and the Luedeking-Piret-Like equation
revealing substrate consumption using MATLAB software, and the optimal parameters and the fermentation kinetic model
were established. Validation experiments revealed the errors between the calculated and experimental values were less than
10%, indicating that the kinetic model can describe the actual fermentation process of P. liquidambari B3.

Key words: endophytic fungus, Phomopsis liquidambari B3, laccase, batch fermentation, kinetic model

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