食品科学

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超声波辅助水酶法提取蚕蛹油工艺优化及脂肪酸组成分析

贾俊强1,2,吴琼英1,2,杜金娟1,何荣海3,桂仲争1,2,颜 辉1,2   

  1. 1.江苏科技大学生物技术学院,江苏 镇江 212018;2.中国农业科学院蚕业研究所,江苏 镇江 212018;
    3.江苏大学食品与生物工程学院,江苏 镇江 212013
  • 出版日期:2014-08-25 发布日期:2014-08-25
  • 基金资助:

    江苏省自然科学基金项目(BK2012693;BK2012708);公益性行业(农业)科研专项(201403064)

Optimization of Ultrasonic-Assisted Aqueous Enzymatic Extraction and Fatty Acid Composition of Silkworm Pupae Oil

JIA Jun-qiang1,2, WU Qiong-ying1,2, DU Jin-juan1, HE Rong-hai3, GUI Zhong-zheng1,2, YAN Hui1,2   

  1. 1. School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China;
    2. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, China;
    3. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
  • Online:2014-08-25 Published:2014-08-25

摘要:

为开发蚕蛹油提取新工艺,以蚕蛹为原料,采用超声波辅助中性蛋白酶水解法提取蚕蛹油。在单因素试验的基础上,以蚕蛹油提取率为响应值,用响应面分析法研究超声功率、底物质量浓度和加酶量对蚕蛹油提取率的影响,并通过气相色谱对蚕蛹油的脂肪酸组成进行分析。结果表明:各因素对蚕蛹油提取率影响从大到小依次为:底物质量浓度、加酶量、超声功率。超声波辅助水解法提取蚕蛹油的最优工艺条件为:底物质量浓度68.2 g/L、超声功率121.6 W、加酶量2 849.4 U/g。在此条件下,蚕蛹油提取率为(90.2±0.66)%。气相色谱分析表明,超声波辅助水酶法与传统溶剂(正己烷)提取的蚕蛹油中脂肪酸组成与含量基本一致,均主要由棕榈油酸、软脂酸、亚麻酸、亚油酸、油酸和硬脂酸组成。

关键词: 超声波, 水酶法, 蚕蛹油, 脂肪酸组成

Abstract:

As a new extraction technique, ultrasonic-assisted neutral protease hydrolysis (UANPH) was used to extract oil
from silkworm pupae. On the basis of single factor experiments, effects of ultrasonic power, substrate concentration and
enzyme dosage on the extraction yield of silkworm pupae oil were studied with response surface methodology. In addition,
the fatty acid composition of silkworm pupae oil was analyzed by gas chromatography (GC). The results showed that the
the extraction yield of silkworm pupae oil was affected in decreasing order by substrate concentration, enzyme dosage and
ultrasonic power. The optimized operating parameters were as follows: substrate concentration 68.2 g/L, ultrasonic power
121.6 W and enzyme dosage 2 849.4 U/g. Experiments carried out under the optimal conditions led to an extraction yield
of (90.2 ± 0.66)%. GC analysis showed that composition and contents of fatty acids in silkworm pupae oils by UANPH
extraction and traditional solvent (n-hexane) extraction were in substantial agreement. The silkworm pupae oils were mainly
composed of palmitoleic acid, palmitic acid, linolenic acid, linoleic acid, oleic acid and stearic acid.

Key words: ultrasound, aqueous enzymatic extraction, silkworm pupae oil, fatty acid composition

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