关键词: 氧化魔芋葡甘露聚糖, 齐口裂腹鱼, 品质, 过氧化物酶体增殖物激活受体-&alpha, 脂肪酸结合蛋白

Abstract:

Objective: To study the effect of dietary oxidized konjac glucomannan (OKGM) on the quality and lipid metabolismrelated
gene expression of Schizothorax prenanti Tchang (SPT). Methods: The SPT with body weight of approximately 80 g were
randomly divided into five groups which were fed 0 (control group), 0.4%, 0.8%, 1.6% and 3.2% (experimental groups) OKGM
for 60 days, respectively. The results showed that compared with the control group, the protein content in abdominal muscle of SPT
was significantly increased by adding 1.6% and 3.2% OKGM in the SPT diet (P ＜ 0.05), the fat content in abdominal muscle was
significantly reduced by adding 0.8% OKGM in the diet (P ＜ 0.05); however, the amount of OKGM had no significant impact
on muscle moisture or crude ash in SPT (P ＞ 0.05). In addition, no significant difference in pH, dehydration rate, hydroxyproline
content or collagen content in abdominal muscle of SPT was observed in response to varying amounts of OKGM (P ＞ 0.05).
Meanwhile, PPAR-α mRNA expression level in muscle of SPT was significantly increased by dietary addition of 1.6% OKGM
(P ＜ 0.05), and a significant increase in this parameter was also observed for liver of SPT administered with 0.8% OKGM
(P ＜ 0.05). In contrast, FABP mRNA expression level in muscle of SPT was significantly decreased by dietary addition of
0.8% OKGM (P ＜ 0.01), yet the amount of OKGM had no significant impact on the expression level of FABP mRNA in liver (P ＞ 0.05).
Conclusion: The dietary addition of OKGM can improve the quality of SPT and regulate the gene expression associated with lipid metabolism.

Key words: oxidized konjac glucomannan, Schizothorax prenanti Tchang, quality, peroxisome proliferator activated receptor-α, fatty acid-binding protein