发酵芝麻粕小肽体内抗氧化和免疫活性

doi:10.7506/spkx1002-6630-201419050

食品科学

发酵芝麻粕小肽体内抗氧化和免疫活性

李旸，张莉，陈文帮，钱坤，李吕木，徐雅芫，许发芝，丁小玲

1.安徽农业大学茶与食品科技学院，安徽合肥 230036； 2.安徽省兽药饲料监察所，安徽合肥 230022；
3.安徽省畜牧生物工程技术研究中心，安徽合肥 230031；4.安徽农业大学动物科技学院，安徽合肥 230036

出版日期:2014-10-15 发布日期:2014-10-17

in vivo Antioxidant Activity and Immune Activity of Sesame Peptides from Solid-State Fermentation

LI Yang, ZHANG Li, CHEN Wen-bang, QIAN Kun, LI Lü-mu, XU Ya-yuan, XU Fa-zhi, DING Xiao-ling

1. College of Tea and Food Science and Technology, Anhui Agricultural University, Hefei 230036, China;
2. Institute of Anhui Animal Feed and Veterinary Drug Control, Hefei 230022, China;
3. Anhui Animal Biological Engineering Technology Research Center, Hefei 230031, China;
4. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Online:2014-10-15 Published:2014-10-17

摘要/Abstract

摘要：

研究从发酵芝麻粕中提取分离出的小肽体内抗氧化和免疫活性。将56 只4 周龄健康昆明小鼠随机分成7 组，分别灌胃生理盐水，低、中、高3 个剂量的小肽（三肽和四肽），连续灌胃28 d后取血，测定肝脏、脾脏、胸腺系数，血清中丙二醛含量，肝匀浆中的丙二醛含量、超氧化物歧化酶与谷胱甘肽过氧化物酶的活性，脾脏中T淋巴细胞CD3＋、CD4＋和CD8＋数量。灌胃发酵芝麻粕三肽和四肽后，血清和肝脏中丙二醛含量显著下降（P＜0.05），肝脏中超氧化物歧化酶和谷胱甘肽过氧化物酶活性显著升高（P＜0.05），四肽高剂量组小鼠的CD4＋/CD8＋值显著升高（P＜0.05）。结果表明发酵芝麻粕三肽和四肽均具有体内抗氧化能力，四肽在本实验设定中的高剂量条件下能在一定程度上增强机体的免疫活性。

关键词: 发酵芝麻粕, 小肽, 抗氧化, 免疫活性, 动物实验

Abstract:

The antioxidant activity and immune activity of small peptides (tripeptides and tetrapeptides) from fermented
sesame meal were investigated in vivo. Totally 56 healthy Kunming mice (4 weeks old) were randomly divided into 7
groups. The mice in normal control group were administered with saline, and those in test groups were administered
with sesame tripeptides or tetrapeptides at doses of 2, 4, and 8 mg/d, respectively. After continuous intragastric admi
nistration for 28 days, the mice were sacrificed to harvest serum and liver. The organ coefficients of liver, spleen and
thymus, malondialdehyde (MDA) content in serum and liver, and the activities of superoxide dismutase (SOD) and
glutathione peroxidase (GSH-Px) in liver were determined. T-lymphocytes such as CD3＋, CD4＋ and CD8＋ in spleen
were tested by flow cytometer. The results showed that, in mice fed the sesame peptides, the organ coefficients were not
changed significantly (P ＞ 0.05), but MDA contents in serum and liver were reduced significantly (P ＜ 0.05). SOD and
GSH-Px activities in liver exhibited a significant increase. CD4＋/CD8＋ ratio was also significantly increased (P ＜ 0.05) in
the tetrapeptide high-dose group. Therefore, both tripeptides and tetrapeptides from fermented sesame meal have obvious
antioxidant capacity in vivo, and the tetrapeptides can enhance the body’s immune activity when administered at high dose.

Key words: fermented sesame meal, peptide, antioxidant activity, immune activity, animal experiments

中图分类号:

TS201.4

李旸，张莉，陈文帮，钱坤，李吕木，徐雅芫，许发芝，丁小玲. 发酵芝麻粕小肽体内抗氧化和免疫活性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201419050.

LI Yang, ZHANG Li, CHEN Wen-bang, QIAN Kun, LI Lü-mu, XU Ya-yuan, XU Fa-zhi, DING Xiao-ling. in vivo Antioxidant Activity and Immune Activity of Sesame Peptides from Solid-State Fermentation[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201419050.

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发酵芝麻粕小肽体内抗氧化和免疫活性

in vivo Antioxidant Activity and Immune Activity of Sesame Peptides from Solid-State Fermentation

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