食品科学

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核桃凝集素的提取及其性质

冀爱青1,韩红艳1,杨红雁2,吴国良3   

  1. 1.晋中学院生物科学与技术学院,山西 晋中 030600;2.晋中市气象局,山西 晋中 030600;
    3.河南农业大学园艺学院,河南 郑州 450002
  • 出版日期:2014-10-25 发布日期:2014-11-07
  • 通讯作者: 冀爱青 E-mail:aiqingji2003@163.com
  • 基金资助:

    国家自然科学基金面上项目(31171943);山西省特色专业建设项目(晋教材(2012)151号)

Extraction and Characterization of Walnut (Juglans regia) Kernel Lectin

JI Ai-qing1, HAN Hong-yan1, YANG Hong-yan2, WU Guo-liang3   

  1. 1. Department of Biology Science and Technology, Jinzhong College, Jinzhong 030600, China;
    2. Jinzhong City Meteorology Bureau, Jinzhong 030600, China;
    3. College of Horticultural Science, Henan Agricultural University, Zhengzhou 450002, Ch
  • Online:2014-10-25 Published:2014-11-07
  • Contact: JI Ai-qing1 E-mail:aiqingji2003@163.com

摘要:

以核桃仁为材料,对核桃凝集素的分离条件及其部分性质进行研究。结果表明:凝集活性值能准确反映凝集素的血凝效果;核仁经PBS缓冲液(pH 7.4)浸提24 h及质量分数70%硫酸铵分级沉淀,得到的核桃凝集素粗品能凝集人A、B、O、AB型血红细胞,且无血型专一性,其凝集活性不能被常见的单糖和寡糖抑制,但可被糖的衍生物所抑制;凝集活性在30~50 ℃基本不变,有较广泛的pH值作用范围(5.0~8.0);供试的5 个核桃品种中,薄壳香粗提液的凝集活性最强,凝集活性值达到0.478。

关键词: 核桃, 凝集素, 活性, 性质

Abstract:

The aim of this study was to investigate the extraction conditions and some characteristics of lectin from walnut(Juglans regia) kernel. The results indicated that the agglutinating activity (Aa) could accurately reflect blood agglutinationefficiency. Crude lectin with strong hemagglutination activity was obtained from walnut kernel by extraction with phosphate buffer solution (PBS) at pH 7.4 for 24 h followed by fractional precipitation with 70% (NH4)2SO4. The lectin could agglutinate A, B, O and AB types of red blood cells without blood type specificity. The hemagglutination activity could be inhibited by saccharide derivatives, but not by monose or oligose. The lectin from walnut kernel was temperature-insensitive,and showed a broad reaction pH range (5.0–8.0). Thehemagglutination activity of walnut kernel lectin from Baokexiang cultivar was the strongest among five tested walnut cultivars, which showed an Aa of 0.478.

Key words: walnut (Juglans regia), lectin, activity, characterization

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