关键词: 小麦秸秆, 低聚木糖, 抗氧化活性

Abstract:

The enzymatic hydrolysis of wheat straw for preparing xylooligosaccharides using xylanase was optimized using
an orthogonal array design, and the xylooligosaccharides obtained were assessed for antioxidant activity. A hydrolysis
temperature and 4 h hydrolysis at an enzyme concentration of 3 g/L and a substrate concentration of 70 g/L with an
initial pH of 6.0 proved optimal for the preparation of xylooligosaccharides. The optimized conditions yielded 9.5 g/L
of reducing sugar and 26.3 g/L of soluble sugar in the product with an average degree of polymerization of 2.77. The
xylooligosaccharides showed dose-dependent radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH),
hydroxyl and superoxide anion radicals.

Key words: wheat straw, xylooligosaccharides, antioxidant activity