关键词: 火腿, 高效液相色谱串联质谱, 同位素内标法, 喹诺酮类

Abstract:

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) was developed for
simultaneous determination of 14 quinolone residues (QNS) in ham. Ham sample was extracted with acetonitrile, defatted
with hexane saturated with acetonitrile and cleaned up by MAX solid phase extraction. The mass spectrometer was operated
in the positive ion mode using select reaction monitoring for qualitative and quantitative analysis. Isotope internal standard
was used for quantitative analysis. The limit of detection of the method was 0.6 μg/kg. Mean recovery rates for 14 QNS in
a negative ham sample spiked at three levels were between 70% and 115%, with RSD ranging from 1.2% to 15.6%. This
method proved to be of simplicity, high sensitivity and good selectivity. It has been used for routine test in our laboratory.

Key words: ham, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS), isotopic internal standard method, quinolone (QNS)