食品科学

• 分析检测 • 上一篇    下一篇

基于电子舌技术检测商业果汁中脂环酸芽孢杆菌

李二虎,冯佳洁,许 灿,潘思轶*   

  1. 环境食品学教育部重点实验室,华中农业大学食品科学技术学院,湖北 武汉 430070
  • 出版日期:2014-11-25 发布日期:2014-12-09
  • 基金资助:

    国家自然科学基金青年科学基金项目(31201414);中央高校基本科研业务费专项(2013PY098)

Detection of Alicyclobacillus sp. Spoilage of Commercial Fruit Juice Beverages by Electronic Tongue

LI Er-hu, FENG Jia-jie, XU Can, PAN Si-yi*   

  1. Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology,
    Huazhong Agricultural University, Wuhan 430070, China
  • Online:2014-11-25 Published:2014-12-09

摘要:

采用法国Alpha M.O.S.公司生产的α-Astree Ⅱ型电子舌对7 种市售果汁饮料及其接种脂环酸芽孢杆菌样品进行测定,所得数据应用主成分分析法进行辨别分析。2 种含乳类果汁饮料未能被区分开,其他5 种样品均可区分。将电子舌可区分的6 种果汁饮料接种两株脂环酸芽孢杆菌并在45 ℃条件下培养30 d,所有接种处理中均可检出脂环酸芽孢杆菌。3 种浑浊型饮料的检出量较多,大于103 CFU/mL。3 种澄清型饮料的检出量较少,小于103 CFU/mL。应用电子舌技术可将6 种接种脂环酸芽孢杆菌DSM 3922的果汁饮料与对照组区分开,4 种接种脂环酸芽孢杆菌XC-6的果汁饮料与对照组区分开,2 种接种脂环酸芽孢杆菌XC-6的混合果汁饮料和橙汁饮料同对照组未能被区分。

关键词: 脂环酸芽孢杆菌, 果汁饮料, 电子舌, 主成分分析

Abstract:

Commercial fruit juice beverages and their samples inoculated with Alicyclobacillus sp. isolates were
discriminated by an α-Astree Ⅱ electronic tongue with principal component analysis. Except for two beverages mainly
containing dairy components, other samples could be separated in different groups. Six beverages that could be discriminated
by E-tongue were inoculated with two Alicyclobacillus sp. isolates and were incubated at 45 ℃ for 30 days. All inoculated
samples were detected with colonies on Bacillus acidoterrestris plate, but number of colonies varied in different beverages.
Three cloudy samples had a higher colony number over 103 CFU/mL and three clear samples had a lower colony number
below 103 CFU/mL. All samples inoculated with Alicyclobacillus sp. DSM 3922 could be distinguished from control by
E-tongue method. Excepted for mixed fruit juice and orange juice, other samples with Alicyclobacillus sp. XC-6 inoculation
could be grouped from the control by E-tongue method.

Key words: Alicyclobacillus sp., fruit juice beverage, electronic tongue, principal component analysis

中图分类号: