食品科学

• 基础研究 • 上一篇    下一篇

植物乳杆菌NDC75017抗氧化活性研究

宋晓辰,彭新颜,李凤梅,姜毓君,于海洋   

  1. 1.鲁东大学食品工程学院,山东 烟台 264025;2.国家乳业工程技术研究中心,黑龙江 哈尔滨 150086;
    3.山东商务职业学院食品工程系,山东 烟台 264670
  • 出版日期:2014-11-15 发布日期:2014-11-06

Antioxidant Activity of Lactobacillus plantarum NDC75017

SONG Xiao-chen, PENG Xin-yan, LI Feng-mei, JIANG Yu-jun, YU Hai-yang   

  1. 1. College of Food Engineering, Ludong University, Yantai 264025, China; 2. National Research Center of Dairy Engineering and
    Technology, Harbin 150086, China; 3. Department of Food Engineering, Shandong Business Institute, Yantai 264670, China
  • Online:2014-11-15 Published:2014-11-06

摘要:

目的:研究酸奶中所筛选的植物乳杆菌(Lactobacillus plantarum)NDC75017在不同体系中的抗氧化作用模式。方法:测定了活细胞、无细胞提取物、胞外分泌物及菌体灭活物质的自由基清除能力、总抗氧化能力(totalantioxidant capacity,T-AOC)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)活性、还原能力和金属离子螯合能力。结果:株菌能够耐受一定浓度的过氧化氢(H2O2),无细胞提取物的超氧阴离子自由基(O2-•)和DPPH自由基清除能力最强,胞外分泌物的羟自由基(•OH)清除作用最好。无细胞提取物和胞外分泌物的T-AOC效果最佳,与另外两组差异显著(P<0.05)。胞外分泌物的GSH-Px和T-SOD酶活性最高,明显比无细胞提取物和菌体灭活组效果好(P<0.05)。4 组样品都具有还原能力和金属离子螯合能力,但Cu2+螯合能力不如Fe2+螯合能力效果好。结论:菌株具有抗氧化活性,其抗氧化本质可能与其具有金属离子螯合能力、提供电子和清除自由基等作用模式有关。

关键词: 植物乳杆菌NDC75017, 抗氧化活性, 作用模式

Abstract:

Objective: The antioxidant activity of Lactobacillus plantarum NDC75017 isolated from traditional yoghurt was
investigated in different systems. Methods: Free radical-scavenging ability, total antioxidant capacity (T-AOC), glutathione
peroxidase (GSH-Px) activity, total superoxide dismutase (T-SOD) activity, reducing power, and metal ion chelating
capability were determined in the intact cells, cell-free extract, extracellular secretion, and inactive cells. Results: The
strain had resistance to hydrogen peroxide, the cell-free extract indicated the strongest superoxide anion and DPPH radical
scavenging capacity, and the extracellular secretion showed the best hydroxyl radical scavenging capacity. Both the latter
two samples exhibited the most potent T-AOC (P < 0.05), showing a significant difference from two other samples. The
extracellular secretion displayed the highest GSH-Px and T-SOD enzyme activities, significantly higher than those of the cell
free extract and inactive cell groups (P < 0.05). All these four samples possessed reducing power and metal ion chelating
capability, more effective for Fe2+ than Cu2+. Conclusion: Lactobacillus plantarum NDC75017 has good antioxidant activity
as a metal ion chelator, electron provider, and radical stabilizer for inhibiting oxidation.

Key words: Lactobacillus plantarum NDC75017, antioxidant activity, action mechanism