食品科学

• 基础研究 • 上一篇    下一篇

中华鳖腿肉蛋白质体内外消化研究

张 丹,王锡昌   

  1. 上海海洋大学食品学院,上海 201306
  • 出版日期:2014-11-15 发布日期:2014-11-06

Digestibility in vitro and in vivo of Chinese Soft-Shelled Turtle Protein

ZHANG Dan, WANG Xi-chang   

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Online:2014-11-15 Published:2014-11-06

摘要:

通过测定氨基酸组成、体内外蛋白质消化率及经蛋白质消化率校正的氨基酸评分(protein digestibilitycorrectedamino acid scores,PDCAAS)等指标,评价热处理前后中华鳖腿肉蛋白质的营养价值。结果表明:中华鳖腿肉热处理前后必需氨基酸含量分别为36.84%、34.55%(以干质量计),占氨基酸总量的41.12%、40.20%,其构成符合联合国粮农组织/世界卫生组织推荐的理想模式。热处理前,测得中华鳖腿肉蛋白质体外消化率为83.68%,体内消化率为95.05%;热处理后,测得其体外消化率为87.04%,体内消化率为96.18%,热处理后消化率显著提高(P<0.05)。结合氨基酸评分及真实消化率,计算得到热处理前其PDCAAS为100%,由于蒸煮过程营养物质的流失,测得热处理后Trp氨基酸评分最低为80,热处理后其PDCAAS为76.94%。

关键词: 中华鳖, 体内消化, 体外消化, 经蛋白质消化率校正的氨基酸评分

Abstract:

The nutritional value of Chinese soft-shelled turtle leg muscle protein before and after heat treatment was
evaluated based on amino composition, in vitro and in vivo digestibility and protein digestibility-corrected amino acid score
(PDCAAS). The results showed that the contents of essential amino acids were 36.84% and 34.55%, respectively before and
after heat treatment. The ratios of total essential amino acids to total amino acids (TEAA/TAA) were 41.12% and 40.20%,
respectively, meeting the FAO/WHO standard. The in vitro and in vivo digestibility of Chinese soft-shelled turtle protein
were 83.68% and 95.05%, and increased to 87.04% and 96.18%, respectively after heat treatment. These results suggested
that heat treatment significantly increased the in vitro and in vivo digestibility (P < 0.05). The PDCAAS of Chinese softshelled
turtle, as calculated by multiplying the limiting amino acid score by protein digestibility, reduced from 100% to
76.94% due to the nutrient losses during cooking.

Key words: Chinese soft-shelled turtle, in vitro digestibility, in vivo digestibility, protein digestibility-corrected amino acid scores (PDCAAS)