食品科学

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低氧胁迫下大豆发芽富集γ-氨基丁酸品种筛选及培养条件优化

王淑芳,杨润强,顾振新   

  1. 南京农业大学食品科技学院,江苏 南京 210095
  • 出版日期:2014-11-15 发布日期:2014-11-06

Cultivar Selection and Culture Condition Optimization for γ-Amino Butyric Acid (GABA) Accumulation in Germinating Soybean under Hypoxia Stress

WANG Shu-fang, YANG Run-qiang, GU Zhen-xin   

  1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Online:2014-11-15 Published:2014-11-06

摘要:

比较理想M-7、95-优1、苏青1号和YH-NJ大豆品种培养4 d后的生理生化变化情况,结果表明:YH-NJ的发芽率、芽长和呼吸强度显著高于其他品种,发芽4 d后YH-NJ的蛋白酶活力、游离氨基酸含量的增加量也显著高于其他品种,γ-氨基丁酸(γ-aminobutyric acid,GABA)产量及增加量均为最大。由此筛选出大豆YH-NJ是发芽富集GABA的良好品种。以YH-NJ为原料,在单因素试验结果的基础上,通过正交试验,得到发芽大豆富集GABA的最佳培养条件为:正常培养4 d、低氧胁迫培养48 h、培养液pH 5.0、发芽温度30 ℃。在此条件下,GABA产量达到1.97 mg/g,是随机组的1.56 倍。

关键词: 大豆, 发芽, &gamma, -氨基丁酸, 富集, 品种筛选, 条件优化, 低氧胁迫

Abstract:

After 4-day germination, the germination percentage, sprout length and respiratory rate of the soybean cultivarYH-NJ were higher than those of all other cultivars tested. The increment of soluble protein, free amino acids and proteaseactivity in YH-NJ sprouts were higher than those of the other cultivars. The content of (GABA) was increased and its levelin YH-NJ sprouts was the highest. Thus, the cultivar YH-NJ was selected for GABA accumulation in further experiments.Using orthogonal design method, the optimal culture conditions for GABA accumulation in 4-day germinated YH-NJ sprouts weredetermined as germination in a culture solution at pH 5.0 for another 48 h under hypoxia stress at 30 ℃. Under these conditions,the content of GABA was 1.97 mg/g DW, which was 1.56 times higher than the control.

Key words: soybean, germination, γ-aminobutyric acid (GABA), accumulation, cultivar selection, condition optimization, hypoxia stress

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