败酱草多糖的组成及抗疲劳与耐缺氧作用

doi:10.7506/spkx1002-6630-201421041

摘要/Abstract

摘要：

目的：评价败酱草多糖（Herba Patriniae polysaccharide，HPP）抗疲劳和耐缺氧的活性。方法：采用水提醇沉法提取HPP，对其基本成分组成进行测定；同时建立小鼠负重游泳模型和耐缺氧模型，同时给予小鼠不同剂量的HPP水溶液，测定小鼠力竭游泳时间与耐缺氧时间以及血乳酸（blood lactic acid，BLA）、血尿素氮的浓度和肌酸激酶（creatine kinase，CK）活性、肝糖原和肌糖原的含量。结果：HPP是一种含蛋白质的多糖，蛋白质含量为13.26%，单糖主要由D-甘露糖、L-鼠李糖、D-半乳糖醛酸、D-葡萄糖、D-半乳糖、L-阿拉伯糖以物质的量比0.53∶0.35∶3.18∶1∶2.72∶1.27组成；服用HPP可使小鼠游泳力竭时间显著延长、肝糖原含量升高，而BLA含量和CK活性均不同程度下降，但耐缺氧时间变化不明显。结论：HPP具有抗疲劳的作用，但其对小鼠的耐缺氧作用不明显。

关键词: 败酱草多糖, 力竭运动, 疲劳, 缺氧

Abstract:

Objective: To evaluate the anti-fatigue and anti-hypoxia effects of Herba Patriniae polysaccharide (HPP).
Methods: HPP was extracted by using the method of water extraction and subsequent alcohol precipitation, and its
main components were determined. A weight loaded swimming model and a hypoxia model in mice were established.
Meanwhile, the mice were given different dose of HPP by oral gavage, and their hypoxia resistance time and swimming
time until exhaustion were determined, and then blood lactic acid (BLA) and blood urea nitrogen (BUN) contents, creatine
kinase (CK) activity and the contents of hepatic glycogen and muscle glycogen were measured. Results: HPP was a
protein-bound polysaccharide composed of D-Man, L-Rha, D-GalUA, D-Glc, D-Gal and L-Ara with a molar ratio of
0.53:0.35:3.18:1:2.72:1.27. HPP could prolong the swimming time until exhaustion of mice and significantly increase
the hepatic glycogen level but decrease the levels of BLA and CK. Conclusion: HPP has an anti-fatigue effect but no antihypoxia
effect in mice.

Key words: Herba Patriniae polysaccharide, exhaustive exercise, fatigue, hypoxia

中图分类号:

G804.7

池爱平，康琛喆，郭欢欢，李虹，王一民，郭飞. 败酱草多糖的组成及抗疲劳与耐缺氧作用[J]. 食品科学, doi: 10.7506/spkx1002-6630-201421041.

CHI Ai-ping, KANG Chen-zhe, GUO Huan-huan, LI Hong, WANG Yi-min, GUO Fei. Composition of Polysaccharides from Herba Patriniae and Their Anti-fatigue and Anti-hypoxia Activities[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201421041.

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败酱草多糖的组成及抗疲劳与耐缺氧作用

Composition of Polysaccharides from Herba Patriniae and Their Anti-fatigue and Anti-hypoxia Activities

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