食品科学

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鼠曲草总黄酮抑制疼痛模型小鼠炎症因子产生而致镇痛作用

黄晓佳,李永金*,李 静,许 潇   

  1. 江苏大学医学院,江苏 镇江 202013
  • 出版日期:2014-11-15 发布日期:2014-11-06

Total Flavonoids from Gnaphalium affine D. Don Exert Analgesic Effect via Inhibiting the Production of Pro-inflammatory Mediators in Mouse Model of Pain

HUANG Xiao-jia, LI Yong-jin*, LI Jing, XU Xiao   

  1. School of Medicine, Jiangsu University, Zhenjiang 202013, China
  • Online:2014-11-15 Published:2014-11-06

摘要:

目的:研究鼠曲草总黄酮的镇痛活性及其作用机制。方法:采用热板刺激、腹腔注射醋酸法构建小鼠疼痛模型,观察灌胃给予不同剂量的鼠曲草总黄酮对小鼠疼痛反应的作用,研究鼠曲草总黄酮对疼痛小鼠血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-6(interleukin-6,IL-6)、一氧化氮(nitric oxide,NO)的作用,以及对腹腔巨噬细胞TNF-α、IL-6、诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)基因表达的影响。结果:鼠曲草总黄酮可明显延长热刺激引起的小鼠舔足时间,抑制腹腔注射醋酸后引起的小鼠扭体反应;降低腹腔注射醋酸致痛小鼠血清中TNF-α、IL-6、NO的含量,也抑制小鼠腹腔巨噬细胞TNF-α、IL-6、iNOS基因表达。结论:鼠曲草总黄酮具有镇痛作用,其作用与减少血清中炎症因子的产生和巨噬细胞介导的炎症反应有关。

关键词: 鼠曲草, 总黄酮, 镇痛, 炎症因子

Abstract:

Objective: To investigate the anti-nociceptive effect of total flavonoids from Gnaphalium affine D. Don and the
involved mechanism. Methods: Employing the mouse model of pain induced by heat and intraperitoneal injection of acetic
acid, the analgesic activity of oral administration of the total flavonoids was assessed. Moreover, the production of cytokines
in mouse plasma, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and nitric oxide (NO) was determined.
The gene expression levels of TNF-α, IL-6 and inducible nitric oxide synthase (iNOS) in peritoneal macrophages were also
examined. Results: The total flavonoids significantly increased the withdrawal latency in hot plate tests and inhibited the
writhing response in mice. In addition, the total flavonoids blocked the production of TNF-α, IL-6, and NO in plasma and
reduced the expression levels of TNF-α, IL-6, and iNOS in the peritoneal macrophages. Conclusions: The total flavonoids
from Gnaphalium affine D. Don exert analgesic effects in mice, which is associated with the inhibition of the production of
cytokines in plasma and macrophage-mediated inflammation.

Key words: Gnaphalium affine D. Don, total flavonoids, analgesic effect, pro-inflammatory mediator

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