食品科学

• 基础研究 • 上一篇    下一篇

茶叶酸性多糖的分离、纯化及其理化性质研究

丁婧思,聂少平*,冯燕玲,王君巧,阙志强   

  1. 南昌大学 食品科学与技术国家重点实验室,江西 南昌 330047
  • 出版日期:2014-12-15 发布日期:2014-12-15

Isolation, Purification and Physico-chemical Properties of Acidic Polysaccharides from Green Tea

DING Jing-si, NIE Shao-ping*, FENG Yan-ling, WANG Jun-qiao, QUE Zhi-qiang   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Online:2014-12-15 Published:2014-12-15

摘要:

采用水提醇沉法从采自江西婺源的粗老绿茶中提取茶叶多糖,通过Sevag法脱蛋白得到精制茶叶多糖,应用高效凝胶渗透色谱法(high performance gel permeation chromatography,HPGPC)分析茶叶多糖纯度并测定其分子质量,进一步测定茶叶多糖的糖含量、蛋白质含量、单糖组成、糖醛酸组成等理化指标,同时对其进行紫外和红外光谱扫描,考察其光谱性质。结果表明:该茶叶多糖组分为均一性高的酸性多糖组分,分子质量约为289 734 D,糖含量为55.1%,蛋白质含量为1.8%。该茶叶多糖酸性组分主要由鼠李糖、核糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖组成,其物质的量比为1.26∶3.18∶4.08∶1.00∶1.52∶3.92,该茶叶多糖酸性组分中含有大量半乳糖醛酸,含量为33.5%。

关键词: 茶叶, 酸性多糖, 理化性质, 单糖组成, 分子质量

Abstract:

Tea polysaccharides (TPS) were extracted and purified from green tea from Wuyuan of Jiangxi province. The
purity and molecular weight (Mw) were determined by high performance gel permeation chromatography (HPGPC). The
contents of sugar and protein, monosaccharide compositions, and uronic acid composition were determined as well. UV
and IR spectroscopy were used for determining their spectroscopic properties. The results showed that the composition was
highly homogeneous acidic polysaccharides. The Mw was 289 734 D, the content of polysaccharide was 55.1%, and the
content of protein was 1.8%. This polysaccharide was mainly composed of rhamnose, ribose, arabinose, mannose, glucose
and galactose with a molar ratio of 1.26:3.18:4.08:1.00:1.52:3.92. The major uronic acid was galacturonic acid with a
relative content of 33.5%.

Key words: tea, acidic polysaccharide, physico-chemical properties, monosaccharide composition, molecular weight

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