枸杞晒干过程中黄酮类化合物和苯丙氨酸解氨酶活性的变化

doi:10.7506/spkx1002-6630-201423016

摘要/Abstract

摘要：

本实验在枸杞表面涂抹Na2CO3、Na2SO3溶液作为除蜡剂，以不涂抹除蜡剂为空白对照，研究晒干过程中总黄酮和苯丙氨酸解氨酶（phenylalanine ammonialyase，PAL）活性的变化，并利用高效液相色谱（highperformance liquid chromatography，HPLC）法检测枸杞中山奈酚、芦丁和槲皮素含量的变化。对总黄酮含量和PAL活性、3 种不同处理方式条件下3 种黄酮类化合物含量之间进行相关性分析。结果表明：Na2CO3、Na2SO3和空白对照处理下的枸杞在晒干过程中PAL活性、总黄酮含量呈现持续上升的趋势，山奈酚、芦丁和槲皮素含量均呈现先增加后下降趋势；同一处理下的PAL活性和总黄酮含量变化呈显著极强正相关，山奈酚、芦丁和槲皮素含量变化呈正相关；除蜡剂影响同一干燥时间下PAL活性、总黄酮、山奈酚、芦丁和槲皮素含量的大小，并且其达到最高值的时间也受除蜡剂的影响。

关键词: 枸杞, 黄酮类化合物, 苯丙氨酸解氨酶, 晒干, 山奈酚, 芦丁, 槲皮素

Abstract:

In this study, sodium carbonate solution and sodium sulfite solution were used as wax removers to smear the
surface of wolfberry (Lycium barbarum L.) fruits and the fruits without wax removal were used as blank control. The
changes in phenylalanine ammonialyase (PAL) activity and total flavonoid content were determined during sun-drying
of wolfberries. Variations in kaempferol, rutin and quercetin contents were also analyzed by high performance liquid
chromatography (HPLC). Meanwhile, correlation analyses were conducted between the content of total flavonoids and PAL
activity, and among the contents of kaempferol, rutin and quercetin. The results showed that continuous accumulations
of PAL activity and total flavonoids were determined during sun-drying. The contents of kaempferol, rutin and quercetin
increased firstly and then decreased. Using the same wax remover, the content of total flavonoids exhibited a significantly
positive correlation with PAL activity. There were also positive correlations among the variations in the contents of
kaempferol, rutin and quercetin for the same treatment. Wax removers affected PAL activity and the contents of total
flavonoid, kaempferol, rutin and quercetin at the same drying time. The time to reach the highest values of total flavonoids,
kaempferol, rutin and quercetin was also influenced by wax removers.

Key words: Lycium barbarum L., flavonoid, phenylalanine ammonialyase, sun-drying, kaempferol, rutin, quercetin

中图分类号:

TS255.1

李朋亮，廖若宇，王旭，龚媛，刘敦华*. 枸杞晒干过程中黄酮类化合物和苯丙氨酸解氨酶活性的变化[J]. 食品科学, doi: 10.7506/spkx1002-6630-201423016.

LI Peng-liang, LIAO Ruo-yu, WANG Xu, GONG Yuan, LIU Dun-hua*. Variations in Flavonoids and PAL Activity from Lycium barbarum L. during Sun-Drying[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201423016.

[1]	王启明，唐瑜婉，李春翼，赵吉春，张宇昊，明建. NaCl浓度对麦醇溶蛋白与槲皮素相互作用的影响[J]. 食品科学, 2021, 42(8): 29-39.
[2]	贾娜，林世文，刘丹，刘登勇. 芦丁诱导猪肉肌原纤维蛋白结构变化对蛋白凝胶特性的改善作用[J]. 食品科学, 2021, 42(8): 67-73.
[3]	谢文，陈华国，赵超，龚小见，周欣. 枸杞多糖的生物活性及作用机制研究进展[J]. 食品科学, 2021, 42(5): 349-359.
[4]	甘小娜，王辉俊，李廷钊，李波. 黑果枸杞化学成分的UPLC-Triple TOF/MS分析及其总花色苷类含量测定[J]. 食品科学, 2021, 42(18): 185-190.
[5]	李朝阳，窦中友，张丽萍，刁静静，江连洲，马萍. 载体油对槲皮素纳米乳液理化稳定性和生物利用度的影响[J]. 食品科学, 2021, 42(12): 85-90.
[6]	贾娜，孙嘉，刘丹，金伯阳，刘登勇. 槲皮素对氧化条件下猪肉肌原纤维蛋白结构及凝胶特性的影响[J]. 食品科学, 2021, 42(10): 45-51.
[7]	韦倩倩，樊金玲，朱文学，白喜婷，任国艳. 植物糖原-槲皮素复合物的制备及特征[J]. 食品科学, 2020, 41(7): 14-22.
[8]	郑梦菲，刘健，屈玮. 6 种5,7-二羟基黄酮对巨噬细胞M1极化的影响[J]. 食品科学, 2020, 41(5): 152-158.
[9]	张静，米佳，禄璐，罗青，闫亚美，冉林武，金波，曹有龙. 黑果枸杞花色苷提取物对胰脂肪酶活性的影响[J]. 食品科学, 2020, 41(5): 8-14.
[10]	王乐飞，古绍彬，吴影. 基于UPLC-MS代谢组学技术的枣果皮黄酮类化合物分析[J]. 食品科学, 2020, 41(24): 155-161.
[11]	王启明，唐瑜婉，杨雅轩，刘士健，赵吉春，张宇昊，明建. pH值对麦醇溶蛋白-槲皮素相互作用及其Pickering乳液特性的影响[J]. 食品科学, 2020, 41(20): 27-34.
[12]	李春翼，王启明，唐瑜婉，张宇昊，赵吉春，明建. 热诱导对麦醇溶蛋白/芦丁相互作用及其乳液流变学特性的影响[J]. 食品科学, 2020, 41(18): 27-34.
[13]	夏丹丹，赵莹莹，马盼盼，王深垒，马常阳，郜晓峰，康文艺. 大肠杆菌DNA定性标准物质的制备及在枸杞子污染检测中的应用[J]. 食品科学, 2020, 41(18): 267-274.
[14]	程晓燕，葛向珍，薛华丽，毕阳. 枸杞鲜果贮藏期间质量损失率与时间的拟合及与质构参数的关系[J]. 食品科学, 2020, 41(17): 261-266.
[15]	韩何丹, 韩照玉, 杜月梅, 刘云帆, 李艾琳, 高丽萍. 枸杞多糖联合顺铂对人肺腺癌细胞A549氧化损伤及凋亡的影响[J]. 食品科学, 2020, 41(15): 194-200.

枸杞晒干过程中黄酮类化合物和苯丙氨酸解氨酶活性的变化

Variations in Flavonoids and PAL Activity from Lycium barbarum L. during Sun-Drying

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价