应用前沿亲和色谱评价5 种知母糖苷类化合物的α-淀粉酶抑制活性

doi:10.7506/spkx1002-6630-201423020

摘要/Abstract

摘要：

以硅胶为载体，制备α-淀粉酶亲和色谱柱，以线扫循环伏安法为检测方法，评价知母提取物的α-淀粉酶抑制活性。首先考察固定化条件对固定化酶的影响以及亲和色谱柱的稳定性。其次利用前沿亲和色谱检测5 种知母糖苷类化合物（新芒果苷、芒果苷、知母皂苷AⅡ、知母皂苷BⅡ、知母皂苷BⅢ）的α-淀粉酶亲和力。最后检测化合物的α-淀粉酶抑制活性。结果表明5 种化合物的α-淀粉酶结合力大小排序为：新芒果苷（Kd＝0.230 5 μmol/L）＞芒果苷（Kd＝0.299 5 μmol/L）＞知母皂苷BⅡ（Kd＝0.312 4 μmol/L）＞知母皂苷BⅢ （Kd＝0.316 2 μmol/L）＞知母皂苷AⅡ（Kd＝0.453 3 μmol/L），其排序结果与样品的α-淀粉酶抑制活性一致。说明前沿亲和色谱可以用来定量检测化合物的α-淀粉酶抑制活性，且可根据亲和力的大小将化合物的抑制活性进行排序。

关键词: 前沿亲和色谱, 线扫循环伏安法, 固定化&alpha, -淀粉酶, &alpha, -淀粉酶抑制活性, 知母

Abstract:

Silica gel was used as the carrier to prepare α-amylase affinity chromatography column. The column was then
used for evaluating the α-amylase inhibitory activity of five glycoside compounds (neomangiferin, mangiferin, timosaponin AⅡ,
timosaponin BⅡ, and timosaponin BⅢ) from Rhizoma anemarrhenae by linear scan cyclic voltammetry. The effect
of immobilization conditions on the immobilized enzyme and the stability of the affinity chromatography column were
investigated. Frontal affinity chromatography was applied to explore the binding affinity of these five glycoside compounds
to α-amylase. Finally, the α-amylase inhibitory activity of samples was assayed. Results showed that the α-amylase
binding affinity of the glycosides followed the decreasing order: neomangiferin (Kd = 0.230 5 μmol/L) > mangiferin
(Kd = 0.299 5 μmol/L) > timosaponin BⅡ (Kd = 0.312 4 μmol/L) > timosaponin BⅢ (Kd = 0.316 2 μmol/L) > timosaponin
AⅡ (Kd = 0.453 3 μmol/L). The same ranking was observed for α-amylase inhibitory activity. Therefore, frontal affinity
chromatography can be used to quantitatively detect the α-amylase inhibitory activity of five glycoside compounds and the
α-amylase inhibitory activity follows the same order as the binding affinity.

Key words: frontal affinity chromatography, linear scan cyclic voltammetry, α-amylase immobilization, α-amylase inhibitory activity, Rhizoma anemarrhenae

中图分类号:

R932

胡园园，郭辉，钱俊青. 应用前沿亲和色谱评价5 种知母糖苷类化合物的α-淀粉酶抑制活性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201423020.

HU Yuan-yuan, GUO Hui, QIAN Jun-qing. Application of Frontal Affinity Chromatography for Evaluating α-Amylase Inhibitory Activity of Five Glycoside Compounds Extracted from Rhizoma anemarrhenae[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201423020.

[1]	王丽波，高婧宇，李腾飞，李莲玉，刘博，张洪龑，杨昱，徐雅琴. 硒化蒲公英多糖的制备、结构表征及益生菌促增殖活性[J]. 食品科学, 2021, 42(7): 169-175.
[2]	曾静，郭建军，涂熠坤，袁林. 嗜热酸性生淀粉α-淀粉酶Gt-amy的C末端结构域功能及生淀粉结合位点分析[J]. 食品科学, 2020, 41(6): 108-115.
[3]	王静，刁翠茹，王华丽，李祥，汪名春，王浩. 鼠尾草酸对α-淀粉酶的抑制作用[J]. 食品科学, 2020, 41(3): 12-17.
[4]	苏娜，伊丽，吉日木图. 驼乳蛋白α-淀粉酶抑制活性肽的制备与鉴定[J]. 食品科学, 2020, 41(22): 148-157.
[5]	侯粲，杜昱光，王曦，肖杰，范怡航，董志忠，常国生，王伟，李颂，应剑. 发酵陈皮黑茶的化学成分差异及体外活性[J]. 食品科学, 2020, 41(18): 226-232.
[6]	曾静，郭建军，涂熠坤，魏国汶，袁林. 嗜热酸性生淀粉α-淀粉酶Gt-amy中结构域C的环化重排及其对Gt-amy催化性能的影响[J]. 食品科学, 2019, 40(20): 144-151.
[7]	徐艳阳，仇洋，王君旸，张铁华，颜伟强，史成军. 黑果腺肋花楸多酚的抑菌效果及对α-淀粉酶活性的抑制作用[J]. 食品科学, 2018, 39(19): 51-57.
[8]	袁林，曾静，郭建军，郭浩，杨罡，陈俊. 极端嗜热酸性α-淀粉酶PFA在枯草芽孢杆菌中的高效分泌表达[J]. 食品科学, 2018, 39(18): 100-108.
[9]	曾静，郭建军，袁林. N-糖基化修饰对极端嗜热酸性α-淀粉酶ApkA酶学性质的影响[J]. 食品科学, 2017, 38(6): 48-54.
[10]	龚凌霄，曹文燕，张英，张慧娟，刘英丽，王静. 青稞麸皮提取物抑制α-葡萄糖苷酶活性研究及成分分析[J]. 食品科学, 2017, 38(6): 179-184.
[11]	李鹏程，朴春红，张岚，张羽，李想，赵梓瀛，王玉华，刘俊梅，于寒松. 荞麦壳黄酮提取物对2型糖尿病大鼠的血糖改善作用及机制[J]. 食品科学, 2017, 38(5): 244-250.
[12]	张洪志，徐庆阳，曹华杰，白芳，陈宁，白钢. α-淀粉酶抑制剂生物合成途径和代谢流分析[J]. 食品科学, 2017, 38(4): 118-124.
[13]	王海丽，李立，陈默，杨春香，王婧，余晨. 气相色谱-质谱联用法检测乙烯-乙烯醇活性食品包装膜中的香叶醇和α-松油醇[J]. 食品科学, 2017, 38(2): 306-310.
[14]	曾静，郭建军，袁林. 定点突变提高极端嗜热α-淀粉酶ApkA的高温活性和热稳定性[J]. 食品科学, 2017, 38(2): 20-26.
[15]	郭雅靖，颜梦婷，林圣楠，杨雪娜，付才力，黄德建. 几种原花青素的降血糖作用及与常见食品原料的结合研究[J]. 食品科学, 2017, 38(19): 156-163.

应用前沿亲和色谱评价5 种知母糖苷类化合物的α-淀粉酶抑制活性

Application of Frontal Affinity Chromatography for Evaluating α-Amylase Inhibitory Activity of Five Glycoside Compounds Extracted from Rhizoma anemarrhenae

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价