食品科学

• 基础研究 • 上一篇    下一篇

苹果ACO1基因及转录因子MdHB-1基因的表达模式

朱 敏,尹明安*,叶红红,任小林   

  1. 西北农林科技大学园艺学院,陕西 杨凌 712100
  • 发布日期:2014-12-15

Expression Patterns of ACO1 Gene and Transcription Factor MdHB-1 in Apple

ZHU Min, YIN Ming-an*, YE Hong-hong, REN Xiao-lin   

  1. College of Horticulture, Northwest A&F University, Yangling 712100, China
  • Published:2014-12-15

摘要:

研究苹果MdACO1基因及转录因子MdHB-1基因在不同器官和不同发育阶段的表达量及其对外源乙烯的反应,以期探讨两基因之间的可能关系。以苹果品种“皇家嘎拉”为试材,利用实时荧光定量聚合酶链式反应技术分析不同器官和果实不同发育阶段基因的表达,以及乙烯利处理果实对基因表达的影响。在不同组织器官中,MdHB-1在花瓣中表达量较高,而MdACO1在成熟果中表达量较高。在果实不同发育阶段中MdHB-1在未成熟果中(盛花期40 d后)表达量最高,MdACO1在成熟果中尤其是花后100 d表达量最高。外源乙烯利(500 mg/L)处理的果实放置0~1 d时抑制MdHB-1的表达,但在7 d后其表达量增高;MdACO1表达量在外源乙烯利处理后迅速升高,并随着放置时间的延长而大幅度增高。转录因子MdHB-1可能参与了苹果幼嫩果实和花器官中其他相关基因表达的调控,这一过程似与MdACO1无关。MdACO1参与了果实的成熟过程,但并无受MdHB-1调控的迹象。外源乙烯会诱导成熟果实ACO1基因的过量表达,这一反馈调节过程似与MdHB-1无关。

关键词: 苹果, ACO基因, 转录因子MdHB-1, 表达模式, 乙烯

Abstract:

In this study, we investigated the expression patterns of the MdACO1 gene and its transcription factor MdHB-1
in different organs at different developmental stages in apple, as well as their response to exogenous ethylene treatment,
with the purpose to reveal the relationship between these genes. The expression levels of MdACO1 and MdHB-1 in apple
cultivar “Royal Gala” were detected in different organs at different fruit development stages, also under exogenous ethylene
treatment by real-time quantitative PCR. Results showed that in different organs, MdHB-1 showed relatively higher
expression level in petal, while MdACO1 exhibited relatively high expression level in mature flesh. At the different stages
of fruit development, MdHB-1 gene was expressed highest in young flesh (about 40 days after the full flowering stage),
but MdACO1 gene exhihibited the highest expression level in mature fruits, specifically at 100 days after the full flowering
stage). Under exogenous ethylene treatment, the expression of MdHB-1 was inhibited in the fruits stored for 0–1 d, but its
expression level increased after 7 d storage, while the expression level of MdACO1 gene increased rapidly with the extension
of time. These results suggested that MdHB-1 may be involved in the regulation network of gene expression in young apple
fruits and floral organs, while MdACO1 was not involved in these processes. The MdACO1 gene played an important role in
the maturation of apple fruits, which was not regulated by MdHB-1. In addition, exogenous ethylene could induce the high
expression of MdACO1 in “Royal Gala”, while MdHB-1 did not participate in this feedback regulation process.

Key words: apple, ACO gene, transcription factor MdHB-1, expression patterns, ethylene

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