食品科学

• 工艺技术 •    下一篇

沙葱总黄酮提取工艺优化及其体外抗氧化、抗菌作用

萨茹丽1,木其尔1,王翠芳1,包玲玲1,敖长金1,*,王思珍2   

  1. 1.内蒙古农业大学动物科学学院,内蒙古 呼和浩特 010018;2.内蒙古民族大学动物科学技术学院,内蒙古 通辽 028000
  • 出版日期:2014-12-25 发布日期:2014-12-29
  • 通讯作者: 敖长金
  • 基金资助:

    国家自然科学基金地区科学基金项目(31260558;31160474);“十二五”国家科技支撑计划项目(2013BAD10B04)

Total Flavonoids from Allium mongolicum Regel: Optimization of Extraction Process and Antioxidant and Antibacterial Effects in vitro

SA Ru-li1, MU Qi-er1, WANG Cui-fang1, BAO Ling-ling1, AO Chang-jin1,*, WANG Si-zhen2   

  1. 1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. College of Animal Science and Technology, Inner Mongolia University for the Nationalities, Tongliao 028000, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: AO Chang-jin

摘要:

在单因素试验的基础上,采用响应面方法对纤维素酶辅助提取沙葱总黄酮的工艺参数进行优化,得到最优工艺条件为提取时间4 h、提取温度39 ℃、体系pH 4.3,沙葱总黄酮提取量为5.48 mg/g。通过体外抗氧化及抗菌实验得出,此条件下提取所得沙葱总黄酮具有较好的总还原能力、体外清除DPPH自由基及清除羟自由基的能力,对沙门氏菌具有较好的抑制作用,对大肠杆菌和金黄色葡萄球菌的抑制作用较弱,对绿脓杆菌无抑制作用。

关键词: 沙葱, 总黄酮, 响应面法, 体外抗氧化, 体外抗菌

Abstract:

This report describes the selection and optimization of experimental conditions for cellulase-assisted extraction
of total flavonoids from Allium mongolicum Regel using a combination of single factor experiment and response surface
methodology. It was demonstrated that the optimal extraction parameters were determined to be hydrolysis at 39 ℃ for 4 h
with an initial solvent pH of 4.3, leading to an extraction yield of 5.48 mg/g. Further this study found that the extracted
flavonoids had good total reduction capability and scavenging activities against DPPH and hydroxyl radicals, and exhibited
strong inhibition against Salmonella. However, the inhibitory effect against E. coli and Staphylococcus aureus was weak,
and no inhibition against Pseudomonas aeruginosa was observed.

Key words: Allium mongolicum Regel, flavonoids, response surface methodology, antioxidant activity in vitro, antibacterial activity in vitro

中图分类号: