食品科学

• 工艺技术 • 上一篇    下一篇

酶法富集小黄鱼内脏鱼油中EPA和DHA甘油酯

刘国艳,张田田,王金星,孙贝贝,边 昊,徐 鑫*   

  1. 扬州大学食品科学与工程学院,江苏 扬州 225127
  • 出版日期:2014-12-25 发布日期:2014-12-29
  • 通讯作者: 徐 鑫

Lipase-Catalyzed Enrichment of EPA and DHA Glycerides from Visceral Oil of Small Yellow Croaker

LIU Guo-yan, ZHANG Tian-tian, WANG Jin-xing, SUN Bei-bei, BIAN Hao, XU Xin*   

  1. College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: XU Xin

摘要:

目的:以小黄鱼内脏精炼鱼油为原料,通过脂肪酶选择性水解法富集二十碳五烯酸(eicosapentaenoic acid,EPA)甘油酯和二十二碳六烯酸(docosahexaenoic acid,DHA)甘油酯。方法:采用气相色谱峰面积外标法定量测定EPA和DHA甘油酯总含量。通过单因素和响应面试验,考察反应时间、pH值、反应温度、加酶量等因素对富集效果的影响。结果:确定最佳工艺条件为反应时间4 h、pH 8.0、反应温度30 ℃、加酶量1.0%,在此条件下,EPA和DHA甘油酯总含量为21.65%,且4 个因素对EPA和DHA甘油酯富集的影响依次增强。结论:富集后鱼油理化指标和感官评价均优于精炼鱼油,EPA和DHA甘油酯总含量是富集前的1.74 倍,获得了天然的EPA和DHA甘油酯。

关键词: 脂肪酶, 小黄鱼内脏, 精炼鱼油, EPA甘油酯, DHA甘油酯

Abstract:

Objective: The refined fish oil extracted from gut organs of small yellow croakers was hydrolyzed by lipase to
enrich eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) glycerides. Methods: Gas chromatography (GC)
combined with peak area external standard method was used to determine the contents of EPA and DHA glycerides. Onefactor-
at-a-time method followed by response surface analysis was used to analyze the effects of pH, temperature, enzyme
amount and hydrolysis time on enrichment efficiency. Results: The optimal conditions for enriching EPA and DHA
glycerides were found to be hydrolysis at 30 ℃ for 4 h with an enzyme dosage of 1.0% under neutral pH condition. Under
these conditions, the total amount of EPA and DHA glycerides was 21.65%, which was influenced in increasing order by
hydrolysis time, pH, temperature and enzyme dosage. Conclusion: The physical and chemical indexes and sensory evaluation
of EPA and DPA-enriched fish oil were better than those of fish oil before enrichment with a 1.74-fold increase in the total
of EPA and DHA glycerides. As a result, the natural EPA and DHA glycerides were obtained.

Key words: lipase, small yellow croaker viscera, refined fish oil, EPA glyceride, DHA glyceride

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