食品科学

• 工艺技术 • 上一篇    下一篇

凝胶层析法分离猪股骨降血压肽及其体外稳定性

舒一梅,李 诚*,付 刚,陈姝娟,何 利,杨 勇,胡 滨,张志清,苏 赵   

  1. 四川农业大学食品学院,四川 雅安 625014
  • 出版日期:2014-12-25 发布日期:2014-12-29
  • 通讯作者: 李 诚

Purification of Antihypertensive Peptides from Porcine Femoral Collagen by Gel Permeation Chromatography and Their Stability in vitro

SHU Yi-mei, LI Cheng*, FU Gang, CHEN Shu-juan, HE Li, YANG Yong, HU Bin, ZHANG Zhi-qing, SU Zhao   

  1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: LI Cheng

摘要:

将猪股骨酶解液超滤分离后,对分子质量小于5 kD的酶解滤液(IC50值为1.362 mg/mL)进行凝胶层析分离,研究洗脱液、流速、上样量3 个因素对猪股骨降血压肽分离纯化效果的影响。结果表明:以去离子水为洗脱液,流速0.6 mL/min,上样量体积分数1%时,能够得到最佳分离效果,其最高活性组分IC50值达到0.401 6 mg/mL。研究凝胶层析分离后抑制率最高的组分的稳定性,发现其有较好的耐热和耐酸碱、耐盐性,在pH 2~6中有良好的溶解性。

关键词: 猪股骨酶解液, 降血压肽, 凝胶层析, 稳定性, 血管紧张素转换酶半抑制浓度

Abstract:

After the enzymatic hydrolysate of porcine femoral collagen prepared by a three-stage hydrolysis procedure
sequentially with alkaline protease, papain and flavourzyme was ultrafiltrated, the fraction with molecular weight below
5 kD, showing an IC50 of 1.362 mg/mL was purified by gel permeation chromatography. The effects of elution solvent,
flow rate and sample loading amount on purification efficiency were examined. The best separation results were achieved
by loading 1% (by volume) of the sample onto the column followed by elution with deionized water at a flow rate of
0.6 mL/min. The eluate fraction with the highest antihypertensive activity, showing an IC50 of 0.401 6 mg/mL, was
harvested. The stability of the antihypertensive peptides was evaluated, and the results demonstrated its good tolerance to
heat, acid and alkali and high solubility at pH 2-6.

Key words: enzymatic hydrolysate of porcine femoral collagen, antihypertensive peptides, gel permeation chromatography, stability, half inhibitory concentration against ACE

中图分类号: