食品科学

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不同产地皱盖疣柄牛肝菌中矿质元素
含量测定及特征分析

李杰庆1,杨天伟1,王元忠2,李 涛3,刘鸿高1,*   

  1. 1.云南农业大学农学与生物技术学院,云南 昆明 650201;2.云南省农业科学院药用植物研究所,云南 昆明 650200;
    3.玉溪师范学院资源环境学院,云南 玉溪 653100
  • 出版日期:2014-12-25 发布日期:2014-12-29
  • 通讯作者: 刘鸿高
  • 基金资助:

    国家自然科学基金地区科学基金项目(31260496;31160409);
    云南省自然科学基金项目(2011FB053;2011FZ195);云南省教育厅科学研究基金项目(2012Y380;2013Z074)

Determination and Characteristic Analysis of Mineral Elements in Leccinum rugosiceps from Different Geographic Origins

LI Jie-qing1, YANG Tian-wei1, WANG Yuan-zhong2, LI Tao3, LIU Hong-gao1,*   

  1. 1. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China;
    2. Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Kunming 650200, China;
    3. College of Resources and Environment, Yuxi Normal University, Yuxi 653100, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: LIU Hong-gao

摘要:

采用微波消解-电感耦合等离子体发射光谱法测定5 个产地皱盖疣柄牛肝菌中10 种矿质元素含量,并用SPSS软件进行方差分析和主成分分析。结果显示:该方法标准物质(GBW 07605)回收率在91%~101%之间,检出限在0.000 1~0.616 5 mg/kg范围,表明该方法准确、可靠;皱盖疣柄牛肝菌中Mg、Cu、Ca、Na、Zn等矿质元素的含量较高,不同产地皱盖疣柄牛肝菌中矿质元素含量具有差异;主成分分析的前3 个主成分累积贡献率达到87.87%,能表达大部分信息,主成分综合得分可以反映出不同产地皱盖疣柄牛肝菌对10 种矿质元素的富集情况。

关键词: 电感耦合等离子体原子发射光谱, 微波消解, 皱盖疣柄牛肝菌, 矿质元素, 主成分分析

Abstract:

The contents of 10 mineral elements in Leccinum rugosiceps from five regions were determined by microwave
digestion-inductively coupled plasma-atomic emission spectrum (ICP-AES), and the obtained data were analyzed by analysis
of variance (ANOVA) and principal component analysis (PCA) using the SPSS software. The results showed that recovery
rates from tea standard (GBW 07605) ranged from 91% to 101% and the detection limits were 0.000 1–0.616 5 mg/kg.
Mg, Cu, Ca, Na and Zn were more abundant in Leccinum rugosiceps. However, there were significant differences among
mushroom samples collected from different regions. PCA showed that the cumulative contribution rate of the first three
principal components was 87.87%, which could express most information. Principal component comprehensive scores could
reflect the enrichment levels of ten mineral elements in Leccinum rugosiceps from different origins.

Key words: inductively coupled plasma-atomic emission spectrum (ICP-AES), microwave digestion, Leccinum rugosiceps, mineral elements, principal component analysis (PCA)

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