食品科学

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肉鸡屠宰场多重耐药沙门氏菌Ⅰ类整合子与磺胺类耐药基因(sul1、sul2和sul3)的检测

赖海梅1,刘书亮1,2,*,邹立扣3,韩新锋1,周 康1,2,朱冬梅1,彭 珍1,李建龙1   

  1. 1.四川农业大学食品学院,四川 雅安 625014;2.四川省农产品加工及贮藏工程重点实验室,四川 雅安 625014;
    3.四川农业大学 都江堰校区微生物学实验室,四川 都江堰 611830
  • 出版日期:2014-12-25 发布日期:2014-12-29
  • 通讯作者: 刘书亮
  • 基金资助:

    公益性行业(农业)科研专项(200903055)

Detection of Integton-1 and Sulphonamide Resistant Genes of Multi-Drug Resistant Salmonella Species Isolated from Broiler Slaughterhouse

LAI Hai-mei1, LIU Shu-liang1,2,*, ZOU Li-kou3, HAN Xin-feng1, ZHOU Kang1,2, ZHU Dong-mei1, PENG Zhen1, LI Jian-long1   

  1. 1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;
    2. Key Laboratory of Agricultural Products Processing and Preservation Engineering of Sichuan Province, Ya’an 625014, China;
    3. Laboratory of Microbiology, Dujiangyan Campus, Sichuan Agricultural University, Dujiangyan 611830, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: LIU Shu-liang

摘要:

目的:了解肉鸡屠宰场多重耐药沙门氏菌Ⅰ类整合子及磺胺类耐药基因携带情况。方法:采用纸片扩散法对肉鸡屠宰场84 株沙门氏菌分离株进行10 种抗生素敏感性实验;应用聚合酶链式反应(polymerase chain reaction,PCR)方法检测多重耐药沙门氏菌sul1、sul2、sul3和int1基因。结果:84 株沙门氏菌对氨苄西林和萘啶酸耐药率高达100%,对环丙沙星、四环素、甲氧苄啶/磺胺甲噁唑、大观霉素、氟苯尼考、庆大霉素耐药率分别为39.29%、35.71%、35.71%、35.71%、22.62%、14.29%。38 株沙门氏菌对3 种及以上抗生素耐药,属于多重耐药菌株。38 株多重耐药菌株中,有20 株携带Ⅰ类整合子。30 株对甲氧苄啶/磺胺甲噁唑耐药的菌株中,均能检测到sul1或sul2或sul3基因,与表型耐药100%符合,其检出率分别为40%、100%、63.3%。结论:肉鸡屠宰场中沙门氏菌耐药现象已不容乐观,沙门氏菌多重耐药性与Ⅰ类整合子的携带之间关系密切,且Ⅰ类整合子在磺胺类耐药菌株的产生中起到重要作用。

关键词: 沙门氏菌, Ⅰ类整合子, 多重耐药性, 磺胺类耐药基因

Abstract:

Objective: To characterize multi-drug resistant Salmonella isolates carrying integeon-1 and sulfonamides-resistant
genes from broiler slaughterhouse. Methods: The susceptibility of 84 Salmonella isolates from broiler slaughterhouse to
10 varieties of antibiotics was evaluated by K-B disc method. In addition, PCR was used to detect the presence of the int1,
sul1, sul2 and sul3 genes of multi-drug resistance in Salmonella isolates. Results: The resistant rates of these 84 Salmonella
isolates were 100% to ampicilin and nalidixic acid, and 39.29%, 35.71%, 35.71%, 35.71%, 22.62% and 14.29% to
ciprofloxacin, tetracycline, benzyl methyl oxygen/sulfamethoxazole, spectinomycin, fluorine benzene nicol and gentamicin,
respectively. It was found that 38 of these isolates exhibited resistance to at least three antibiotics as multi-drug resistant
strains. Among the 38 multi-drug resistant Salmonella strains, 20 carried integron-1. Sulfonamide resistant genes such as
sul1 or sul2 or sul3 genes were detected in 30 sulfonamides resistant Salmonella isolates, suggesting 100% consistency
between the results of drug sensitivity tests and PCR. The detection rates of the sulfonamides resistant genes sul1, sul2 and sul3
genes by PCR were 40%, 100% and 63.3%, respectively. Conclusion: These results implied that the antimicrobial resistance of
Salmonella isolates from broiler slaughterhouse is not optimistic. A close correlation exists between the multi-drug resistance and
the carrier condition of the integron-1, which may play a critical role in the sulphonamide resistance in Salmonella isolates.

Key words: Salmonella, integron-1, multi-drug resistance, sulfonamide-resistant genes

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