食品科学

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粮食及果蔬中叶酸含量分析

邵丽华1,王 莉1,2,*   

  1. 1.山西师范大学生命科学学院 ,山西 临汾 041004;2.山西师范大学现代文理学院生物系,山西 临汾 041004
  • 出版日期:2014-12-25 发布日期:2014-12-29
  • 通讯作者: 王 莉
  • 基金资助:

    2012年度校级大学生创新性实验项目(SD2012CXSY-31);
    山西省引进优秀人才专项(2010-37);山西省回国留学人员科研资助项目(2011-056)

Analysis of Folic acid Contents in Main Grain Crops, Fruits and Vegetables

SHAO Li-hua1, WANG Li1,2,*   

  1. 1. College of Life Science, Shanxi Normal University, Linfen 041004, China;
    2. Department of Biology, College of Modern Arts and Sciences, Shanxi Normal University, Linfen 041004, China
  • Online:2014-12-25 Published:2014-12-29
  • Contact: WANG Li

摘要:

对日常食用的主要粮食及果蔬的叶酸含量进行测定与评价,为人体科学合理补充叶酸提供理论依据。在山西省各农户及市场搜集11 种主要粮食、17 种常见蔬菜、8 种常见水果。粮食样品研磨全部过100 目筛子备用,市售新鲜果蔬样品将可食用部分随机多点采样,切碎混合。食物叶酸用磷酸二氢钾溶液恒温水浴浸提,加苯胺处理过的活性炭吸附,用体积分数3%氨-70%乙醇洗脱,采用高锰酸钾氧化-间接荧光法测定。结果显示,主要食物叶酸含量存在差异,叶酸含量较高的粮食种类有:大豆、花生、绿豆、小米、燕麦、玉米和苦荞,含量均在1.56 μg/g(以干质量计)以上;含量较高的蔬菜有:菠菜、油菜、香菇、油麦菜、生菜、卷心菜、青椒、芹菜、白菜和南瓜,含量均在1.53 μg/g(以湿质量计)以上;人们日常食用的水果叶酸含量均在1.74 μg/g(以湿质量计)以上;只要合理调整膳食结构,粗细搭配,多摄食蔬菜水果,就有望通过食疗来补充人体所缺乏的叶酸。

关键词: 粮食, 水果, 蔬菜, 叶酸含量

Abstract:

In this study, the contents of folic acid in main grain crops, fruits and vegetables were determined and evaluated
to provide a theoretical basis for reasonable supplementation of folic acid for people’s daily consumption. A total of 38
samples, including 11 kinds of main grain crops, 17 kinds of common vegetables and 8 kinds of common fruits, were
collected from some households and local markets in Shanxi Province. Grain samples were prepared by grinding and sieving
through 100 mm sieve, and fresh fruits and vegetables samples were randomly prepared by collecting the edible parts
and were chopped into small pieces. The contents of folic acid in all samples were extracted with potassium dihydrogen
phosphate in water bath. The optimum extraction was achieved by addition of the active carbon adsorbent treated by
aniline to the extraction solvent, the extracts were eluted continuously with a mixture containing 3% ammonia and 70%
ethanol, and indirect fluorescent method was used to determine the fluorescent intensity of folic acid oxidized by potassium
permanganate. Data showed that there was a significant difference in the contents of folic acid between the main grain
crops, which were all over 1.56 μg/g md, and the grain crops containing higher folic acids included soybean, peanut, mung
bean, foxtail millet, oat, corn and buckwheat. The vegetables with higher folic acid content surpassing 1.53 μg/g mf were
in decreasing order: spinach, rapeseed, shiitake, leaf lettuce, lettuce, cabbage, green pepper, celery, Chinese cabbage
and pumpkin. The folic acid content in the fruits for daily consumption was over 1.74 μg/g mf. These data may provide a
scientific basis for reasonable dietary supplementation of folic acid as well as reasonable adjustment in dietary structure
based on intake of fruits and vegetables.

Key words: grain crop, fruit, vegetable, folic acid content

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