气相色谱法检测油脂中低热量短长链甘油三酯

食品科学 ›› 0, Vol. ›› Issue (): 0-0.

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气相色谱法检测油脂中低热量短长链甘油三酯

操丽丽¹,庞敏¹,潘丽军¹,姜绍通²

1. 合肥工业大学生物与食品工程学院
2. 安徽省合肥市合肥工业大学生物与食品工程学院

收稿日期:2014-07-04 修回日期:2014-12-01 出版日期:2015-01-25 发布日期:2015-01-16
通讯作者: 姜绍通 E-mail:jiangshaotong@163.com
基金资助:
国家自然科学基金面上项目;“十一五”国家科技支撑计划项目;合肥工业大学青年教师创新项目

Determination of Short and Long Chain Triglycerides in Oil by Gas Chromatography

Received:2014-07-04 Revised:2014-12-01 Online:2015-01-25 Published:2015-01-16
Contact: Shao-Tong JIANG E-mail:jiangshaotong@163.com

摘要/Abstract

摘要： 利用气相色谱-质谱(GC-MS)联用技术结合计算机质谱库检索和特征离子，对短长链甘油三酯(SLCTs)进行组分鉴定。建立以DB-17ht熔融石英毛细管柱作为分析柱，气相色谱-氢火焰离子化检测器（GC-FID）分析油脂样品中SLCTs含量的方法。结果表明，在质量浓度0.5~20.0 mg/mL范围内，含量与峰面积呈现良好的线性关系；各组分的保留时间和峰面积的RSD值分别在0.05%和2.0%以内，方法的重现性好。加样回收试验表明，平均回收率分别为98.62%和97.59%。该方法可对SLCTs各组分进行了分离，精确度高，重现性好，可用于油脂中SLCTs的含量分析。

关键词: 气相色谱（GC）, 短长链甘油三酯, 质谱（MS）, 氢火焰离子化检测器（FID）

Abstract: The chemical compositions of short and long chain triglycerides (SLCTs) were analyzed by gas chromatography-mass spectrometry (GC-MS) combined with mass spectrometry library retrieval and characteristic ions. A gas chromatography-flame ionization detector (GC-FID) method was developed for determination the content of SLCTs in oil samples when used DB-17ht fused silica capillary column as analytical column. The results showed that there presents a good linear correlation between the peak area and the content at concentration range of 0.5~20 mg/mL. The relative standard deviations for retention time and peak area of each composition in SLCTs were all less than 0.05% and 2.0%, respectively, which showed the method with good reproducibility. Recovery tests indicated that the average recovery was 98.62% and 97.59% respectively. By using the above method established, components of SLCTs were separated with good precision and reproducibility, showed the feasibility of the method in determination of SLCTs in oil and fat.

Key words: gas chromatography(GC), short and long chain triglycerides, mass spectrometry(MS), flame　ionization detector(FID)

中图分类号:

TS227

操丽丽庞敏潘丽军姜绍通. 气相色谱法检测油脂中低热量短长链甘油三酯[J]. 食品科学, 0, (): 0-0.

参考文献

[1]Hayes J R, PENCE D H, SCHEINBACH S, et al.Review of triacylglycerol digestion,absorption,and metabolism with respect to SALATRIM triacylglycerols[J].Journal of agricultural and food chemistry, 1994, 42(2):474-483
[2]OSBORN H T, AKOH C C.Structured lipids–novel fats with medical,nutraceutical,and food applications[J].Comprehensive Reviews in Food Science and Food Safety, 2002, 1(3):110-120
[3]TSUZUKI W.Acidolysis between triolein and short chain fatty acid by lipase in organic solvents[J].Bioscience biotechnology and biochemistry, 2005, 69(7):1256-1261
[4]FOMUSO L B, AKOH C C.Enzymatic modification of triolein: Incorporation of caproic and butyric acids to produce reduced-calorie structured lipids[J].Journal of the American Oil Chemists' Society, 1997, 74(3):269-272
[5]LEE K T, JONES K C, FOGLIA T A.Separation of structured lipids by high performance liquid chromatography[J].Chromatographia, 2002, 55(3):197-201
[6]LEE J H, JONES K C, FOGLIA T A, et al.Separation of triacylglycerol species from interesterified oils by high-performance liquid chromatography[J].Journal of the American Oil Chemists' Society, 2007, 84(3):211-217
[7]CAO Y, QI S J, ZHANG Y, et al.Synthesis of structured lipids by lipase-catalyzed interesterification of triacetin with camellia oil methyl esters and preliminary evaluation of their plasma lipid-lowering effect in mice[J].Molecules, 2013, 18(4):3733-3744
[8]HAN L, XU Z, HUANG J, et al.Enzymatically catalyzed synthesis of low-calorie structured lipid in a solvent-free system: optimization by response surface methodology[J].Journal of Agricultural and Food Chemistry, 2011, 59(23):12635-12642
[9]HUANG A S, ROBINSON L R, GURSKY L, et al.Quantification of generic Salatrim material in foods by the combination of nontraditional crude fat extraction and short nonpolar high temperature capillary gas chromatography[J].Journal of Agricultural and Food Chemistry, 1995, 43(7):1834-1844
[10]HUANG A S, ROBINSON L R, PELLUSO T A, et al.Quantification of generic Salatrim in foods containing Salatrim and other fats having medium- and long-chain fatty acids[J].Journal of Agricultural and Food Chemistry, 1997, 45(5):1770-1778
[11]YANG T H，JANG Y，HAN J J, et al.Enzymatic synthesis of low-calorie structured lipids in a solvent-free system[J].Journal of the American Oil Chemists' Society, 2001, 78(3):291-296
[12]MANGOS T J, JONES K C, FOGLIA T A.Lipase-catalyzed synthesis of structured low-calorie triacylglycerols[J].Journal of the American Oil Chemists’ Society, 1999, 76(10):1127-1132
[13]HUANG A S, ROBINSON L R, GURSKY L, et al.The identification and quantification of Salatrim 23CA in foods by the combination of supercritical fluid extraction,particle peam LC-mass spectrometry,and HPLC with light scattering detector[J].Journal of Agricultural and Food Chemistry, 1994, 42(2):468-473
[14]LEE J H, JONES K C, LEE K T, et al.High-performance liquid chromatographic separation of structured lipids produced by interesterification of macadamia oil with tributyrin and tricaprylin[J].Chromatographia, 2003, 58(9-10):653-658
[15]孟祥河，章银军，毛忠贵.甘油酯的分析方法[J].中国油脂, 2004, 29(1):44-46
[16]张晶，周家春，夏泉明.低热量油脂Salatrim研制[J].粮食与油脂, 2004, 17(1):22-24
[17]朱桃花，范璐，钱向明等.分析植物油脂甘油三酯结构组成的研究现状[J].中国油脂, 2011, 36(5):59-63
[18]王丽丽，汪勇，胡长鹰等.高温气相色谱分析甘油酯的研究[J].中国油脂, 2011, 36(1):75-79
[19]国粮局西安油脂科研院.GB/T 5530-2005 动植物油脂酸值和酸度的测定[S]. 北京: 中国标准出版社, 2005.
[20]罗晓岚.对油脂碱炼工艺的改进[J].中国油脂, 2006, 31(5):17-19
[21]邹建凯.椰子油甘油三酯的高温气相色谱质谱分析[J].分析化学, 2002, 30(4):428-431
[22]邹建凯.奶油甘油三酯的毛细管气相色谱-质谱分析[J].分析测试学报, 2002, 21(5):79-81

气相色谱法检测油脂中低热量短长链甘油三酯

Determination of Short and Long Chain Triglycerides in Oil by Gas Chromatography

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