关键词: 组成分析, 质量评价, 单体皂苷, 正交试验, 高效液相色谱法

Abstract:

Single factor and orthogonal array designs were applied to optimize the conditions for microwave-assisted
extraction of ginsenosides, and the determination of eight main monomer saponins and total saponins in Panax ginseng
extracts was performed by high performance liquid chromatography and spectrophotometry to investigate the influence
of different extraction conditions on the ginsenoside contents and composition in Panax ginseng extracts. The results
showed that 1) the optimum extraction conditions for total saponins were determined as follows: microwave power, 600 W;
extraction temperature, 45 ℃; extraction time, 5 min; the ratio of material to solvent, 1:20; and extraction number, 3; 2) the
optimum extraction conditions for saponin monomers (leading to an extraction yield of 0.98%) were 300 W, 35 ℃, 15 min,
a material-to-solvent of 1∶25, and three extraction cycles; 3) under the optimum conditions, with 80% aqueous methanol as
the extraction solvent, the yield of total saponins was 6.02%, while the total yield of eight ginsenoside monomers was 0.43%
compared to 0.71% with water-saturated butanol as the extraction solvent. In summary, ginseng quality evaluation and
process optimization results were influenced by different extraction methods, extraction conditions and evaluation indexes.

Key words: composition analysis, quality evaluation, monomer saponin, orthogonal array design, high performance liquid chromatography (HPLC)