食品科学

• 工艺技术 • 上一篇    下一篇

响应面法优化甘蔗梢提取物中花青素的分离工艺

李新莹1,姚 舜2   

  1. 1.西南民族大学化学与环境保护工程学院,四川 成都 610041;2.四川大学化学工程学院,四川 成都 610065
  • 出版日期:2015-01-25 发布日期:2015-01-16
  • 基金资助:

    国家自然科学基金青年科学基金项目(81102344);国家自然科学基金面上项目(81373284)

Optimizing Conditions for Separation of Anthocyanins from Sugarcane Tip Extracts Using Response Surface Methodology

LI Xinying1, YAO Shun2   

  1. 1. College of Chemistry and Environment Protection Engineering, Southwest University for Nationalities, Chengdu 610041, China;
    2. School of Chemical Engineering, Sichuan University, Chengdu 610065, China
  • Online:2015-01-25 Published:2015-01-16

摘要:

在单因素试验的基础上,通过中心组合试验设计原理,运用响应面法优化甘蔗梢提取物中花青素成分的综合分离工艺条件。以pH值、洗脱体积和流速为考察因素,进行三因素三水平试验,并获得多元二次回归方程。结果表明,采用大孔吸附树脂从甘蔗梢提取物中分离花青素成分的最佳工艺条件为:将提取浸膏用蒸馏水溶解后调节pH值为5.51,然后以大孔树脂与提取物干质量之比20∶1上样,通过装填有D141型大孔吸附树脂的层析柱,用体积分数为30%的乙醇溶液以1.59 BV/h流速、3.76 BV洗脱体积洗脱。该条件下花青素含量为91 mg/kg,与理论值接近。

关键词: 响应面法, 甘蔗梢, 花青素, 分离

Abstract:

A central composite design was used to investigate the best conditions for separation of anthocyanins from
sugarcane tip extracts. In the separation procedure, the independent variables studied were pH value, elution volume, and
flow rate. Using anthocyanins content as the response variable, a multivariable quadratic regression equation was developed. The
optimal separation conditions for anthocyanins from sugarcane tip extracts were determined as follows: the extract was distilled
with water followed by pH adjustment to 5.51 and loaded onto a column packed with D141-type resin with a resin-to-extract (dry
weight) ratio of 20:1, and then the column was eluted with 3.76 BV of 30% aqueous ethanol at a flow rate of 1.59 BV/h. The
anthocyanins content of the purified product obtained was 91 mg/kg, which was similar to the predicted value.

Key words: response surface methodology, sugarcane tips, anthocyanins, separation

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