食品科学

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响应面法优化蛇足石杉中石杉碱甲的提取工艺

杨 婷1,2,4,武 芸1,2,*,张 泽3,武玉莲3,郑小江1,2,胡世坤2,毛 鑫2   

  1. 1.湖北民族学院 生物资源保护与利用湖北省重点实验室,湖北 恩施 445000;2.湖北民族学院生物科学与技术学院,
    湖北 恩施 445000;3.湖北民族学院附属民大医院,湖北 恩施 445000;4.四川大学生命科学学院,四川 成都 610064
  • 出版日期:2015-01-25 发布日期:2015-01-16
  • 通讯作者: 武 芸
  • 基金资助:

    湖北省教育厅科学技术研究项目(D20141903);湖北省自然科学基金项目(2014CFB615);
    生物资源保护与利用湖北省重点实验室开放基金项目(PKLHB1301);恩施州2013科技研究与开发项目(2013002);
    国家级大学生创新课题项目(201210517030);湖北民族学院大学生创新训练计划项目(2014Z064);
    湖北民族学院林学一级学科(0907)资助项目

Optimization of Extraction Process for Huperzine A from Huperzia serrata

YANG Ting1,2,4, WU Yun1,2,*, ZHANG Ze3, WU Yulian3, ZHENG Xiaojiang1,2, HU Shikun2, MAO Xin2   

  1. 1. Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Hubei Institute for Nationalities,
    Enshi 445000, China; 2. College of Biological Scientific and Technical, Hubei University for Nationalities,
    Enshi 445000, China; 3. University Hospital of Hubei University for Nationalities, Enshi 445000, China;
    4. College of Life Sciences, Sichuan University, Chengdu 610064, China
  • Online:2015-01-25 Published:2015-01-16
  • Contact: WU Yun

摘要:

利用响应面分析法优化恩施州蛇足石杉中石杉碱甲的提取工艺条件。在以提取温度、提取时间、料液比、酒石酸质量分数为影响因素,以石杉碱甲提取量为指标的单因素试验基础上,根据Box-Behnken原理设计三因素三水平的试验分析法,得到提取石杉碱甲最佳工艺条件:料液比1∶30(g/mL)、酒石酸质量分数1.5%、
提取温度41 ℃。采用高效液相色谱法检测石杉碱甲含量,在最优提取条件下,热水浸提4 h,石杉碱甲提取量为11.192 8 mg/100 g。

关键词: 蛇足石杉, 石杉碱甲, 响应面分析法, 高效液相色谱

Abstract:

This paper is concerned with the selection and optimization of important extraction parameters as well as their
levels for the increased yield of huperzine A from the aboveground parts of Huperzia serrata by combined use of single
factor method and response surface methodology (RSM). Solid-to-solvent ratio, solvent concentration and temperature were
identified as main variables that affect extraction efficiency, and their levels were optimized using RSM based on a threevariable,
three-level Box-Behnken design. When the extraction was performed for 4 h under the optimized conditions: 1.5%
tartaric acid as extraction solvent with a solid-to-solvent ratio of 1:30 (g/mL) and an extraction temperature of 41 ℃, the
yield of huperzine A was 11.192 8 mg/100 g.

Key words: Huperzia serrata, huperzine A, response surface analysis, high performance liquid chromatography

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