食品科学

• 营养卫生 • 上一篇    下一篇

玉米蛋白酶解物的解酒作用

马艳秋1,2,郑喜群1,2,*,刘晓兰1,2,孙 琪1   

  1. 1.齐齐哈尔大学食品与生物工程学院,黑龙江 齐齐哈尔 161006;
    2.齐齐哈尔大学 黑龙江省普通高校农产品加工重点实验室,黑龙江 齐齐哈尔 161006
  • 出版日期:2015-01-15 发布日期:2015-01-16

Anti-alcoholism Effects of Corn Protein Hydrolysate

MA Yanqiu1,2, ZHENG Xiqun1,2,*, LIU Xiaolan1,2, SUN Qi1   

  1. 1. College of Food and Biological Engineering, Qiqihar University, Qiqihar 161006, China; 2. Key Constructive Laboratory of
    Processing Agricultural Products of Heilongjiang Province Normal University, Qiqihar University, Qiqihar 161006, China
  • Online:2015-01-15 Published:2015-01-16

摘要:

采用碱性蛋白酶Alcalase和复合蛋白酶Protamex分步对膨化的玉米蛋白粉进行酶解改性,应用动物实验的方法研究玉米蛋白酶解物的解酒作用。结果表明:玉米蛋白酶解物中分子质量小于1 000 u的组分占87.76%,含有较高比例的谷氨酸、亮氨酸和丙氨酸;与酒精模型组相比,双酶酶解得到的玉米蛋白酶解物可使小鼠血液中乙醇含量降低59.43%;在一定时间范围内,玉米蛋白酶解物可以提高小鼠胃中乙醇脱氢酶和肝脏中超氧化物歧化酶活性,降低肝脏中丙二醛含量,增加谷胱甘肽含量。

关键词: 玉米蛋白酶解物, 解酒作用, 双酶分步酶解

Abstract:

The enzymatic hydrolysate of extruded corn gluten produced by sequential hydrolysis with Alcalase and Protamex
was evaluated for its anti-alcoholism effect in mice. The results showed that the hydrolysate contained 87.76% peptides which
molecular weight is less than 1 000 u, and was richer in glutamic acid, leucine and alanine than corn gluten. Gavage administration of
the enzymatic hydrolysate resulted in a 59.43% reduction in blood alcohol concentrations in mice when compared with the model group.
Moreover, this hydrolysate increased alcohol dehydrogenase (ADH) activity in the stomach of mice, and enhanced superoxide dismutase
(SOD) activity and glutataione (GSH) content but reduced malondialdehyde (MDA) content in the liver within a certain time range.

Key words: corn protein hydrolysate, anti-alcoholism effects, sequential two-enzyme hydrolysis

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