食品科学

• 工艺技术 • 上一篇    下一篇

骏枣多糖提取工艺优化及其抗氧化活性

黎云龙1,2,于震宇3,郜海燕1,2,*,杨海龙4,郑 铨5,李 霞6   

  1. 1.南京农业大学食品科技学院,江苏 南京 210095;2.浙江省农业科学院食品科学研究所,
    浙江省果蔬保鲜与加工技术研究重点实验室,浙江 杭州 310021;3.阿克苏职业技术学院,新疆 阿克苏 843000;
    4.温州大学生命与环境科学学院,浙江 温州 325035;5.浙江省诸暨市林业局,浙江 诸暨 311800;
    6.阿克苏茂源果业农民专业合作社,新疆 阿克苏 843000
  • 出版日期:2015-02-25 发布日期:2015-02-16
  • 通讯作者: 郜海燕
  • 基金资助:

    浙江省科技援疆项目(2011C16045)

Optimization of Extraction Process and Antioxidant Capacity of Polysaccharides from Zizyphus jujuba cv. Junzao

LI Yunlong1,2, YU Zhenyu3, GAO Haiyan1,2,*, YANG Hailong4, ZHENG Quan5, LI Xia5   

  1. 1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2. Key Laboratory of Fruits
    and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Food Science Institute,
    Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3. Aksu Vocational and Technical College, Aksu 843000, China;
    4. College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China; 5. Forestry Bureau of Zhuji City,
    Zhuji 311800, China; 6. Specialized Farmers Cooperatives of Aksu Maoyuan Fruit, Aksu 843000, China
  • Online:2015-02-25 Published:2015-02-16
  • Contact: GAO Haiyan

摘要:

研究新疆阿克苏骏枣多糖的提取工艺条件及其抗氧化活性,采用超声波预处理(功率120W、时间30 min、温度60 ℃)辅助热水提取法提取骏枣多糖,以Box-Behnken试验设计结合响应面分析法优化了提取工艺条件,确定最佳工艺参数为热水提取温度83 ℃、液固比17∶1(mL/g)、提取时间4 h。此优化条件下,骏枣粗多糖得率为9.51%。骏枣粗多糖具有清除自由基的作用,当质量浓度为5.0 mg/mL时,对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐自由基和1,1-二苯基-2-苦基肼基自由基的清除率分别达到54.29%和51.43%。

关键词: 响应面法, 骏枣多糖, 超声波, 抗氧化能力

Abstract:

Polysaccharides with antioxidant activity were extracted from Zizyphus jujuba cv. Junzao fruits with hot water
after ultrasonic pretreatment (120 W ultrasonic power, 60 ℃ for 30 min). Important extraction conditions were optimized
by employing response surface methodology based on Box-Behnken design. An extraction temperature of 83 ℃ and 4 h
extraction with a liquid-to-solid ratio of 17:1 (mL/g) were found to be optimal. The extraction yield of crude polysaccharides
under the optimized conditions was 9.51%. The crude polysaccharides from Zizyphus jujuba cv. Junzao had free radical
scavenging capacity, and at a concentration of 5.0 mg/mL, the ABTS+· and DPPH racial scavenging rates were 54.29% and
51.43%, respectively.

Key words: response surface methodology, polysaccharides, ultrasonic, antioxidant capacities

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