转基因大豆MON89788实时荧光PCR检测方法的建立

doi:10.7506/spkx1002-6630-201504038

食品科学

转基因大豆MON89788实时荧光PCR检测方法的建立

刘欣1，张国丛2，周兴虎3，祝长青1，黄明1,*

1.南京农业大学食品科技学院，南京肉制品加工产业创新中心，江苏南京 210095；
2.石家庄市农林科学研究院，河北石家庄 050041；3.南京佳邦食品有限公司，江苏南京 211225

出版日期:2015-02-25 发布日期:2015-02-16
通讯作者: 黄明
基金资助:
公益性行业（农业）科研专项（201303083-2）；江苏省科技支撑计划项目（BE2014304）；南京市生物农业项目（2013）

Establishment of Event-Specific Real-Time PCR Method for Detection of Genetically Modified Soybean MON89788

LIU Xin1, ZHANG Guocong2, ZHOU Xinghu3, ZHU Changqing1, HUANG Ming1,*

1. Nanjing Innovation Center of Meat Products Processing, College of Food Science and Technology,Nanjing Agricultural University, Nanjing 210095, China; 2. Shijiazhuang Academy of Agricultural and Forestry Sciences,Shijiazhuang 050041, China; 3. Nanjing Jiabang Food Co. Ltd., Nanjing 211225, China

Online:2015-02-25 Published:2015-02-16
Contact: HUANG Ming

摘要/Abstract

摘要：

为实现转基因大豆MON89788的标识管理，针对转基因大豆MON89788的品系特异性序列设计引物和TaqMan探针，建立转基因大豆MON89788实时荧光聚合酶链式反应（polymerase chain reaction，PCR）检测方法，并对该方法的特异性、灵敏度和重复性进行检测。结果显示：建立的转基因大豆MON89788实时荧光PCR检测方法能扩增出127 bp的产物，特异性强，灵敏度达到0.1%，约为40 个单倍体基因组拷贝，检测重复性好，可成功应用于实际样品检测。因此，建立的转基因大豆MON89788实时荧光PCR检测方法可以应用于转基因大豆MON89788大豆及其制品的检测。

关键词: 转基因大豆, MON89788品系, 实时荧光PCR, 品系特异性检测, 转基因标识

Abstract:

For implementation of labeling regulations, an event-specific real-time PCR method for the detection of
genetically modified soybean MON89788 was established in this study. Primers and TaqMan probe were designed based on
the event-specific sequence of MON89788 soybean. The specificity, sensitivity and repeatability of the developed method
were examined, respectively. The results showed that the method could amplify a 127 bp product specifically; the limit of
detection was 0.1% in 50 ng of soybean genomic DNA, approximately corresponding to 40 soybean haploid genome copies;
and this method had good repeatability and could be successfully applied to the detection of real samples. These results
indicated that the strain-specific real-time PCR method was suitable for the identification of genetically modified soybean
MON89788 and its products.

Key words: genetically modified soybean, MON89788 event, real-time PCR, event-specific detection, transgenic labeling

中图分类号:

S565.1

刘欣1，张国丛2，周兴虎3，祝长青1，黄明1,*. 转基因大豆MON89788实时荧光PCR检测方法的建立[J]. 食品科学, doi: 10.7506/spkx1002-6630-201504038.

LIU Xin1, ZHANG Guocong2, ZHOU Xinghu3, ZHU Changqing1, HUANG Ming1,*. Establishment of Event-Specific Real-Time PCR Method for Detection of Genetically Modified Soybean MON89788[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201504038.

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转基因大豆MON89788实时荧光PCR检测方法的建立

Establishment of Event-Specific Real-Time PCR Method for Detection of Genetically Modified Soybean MON89788

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