关键词: 乙二胺四乙酸二钠, 超高效液相色谱-串联质谱, 八宝粥罐头

Abstract:

An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method was developed
for the determination and confirmation of ethylenediaminetetraacetic acid disodium (EDTA-2Na) residues in canned
eight-ingredient porridge. The EDTA-2Na residues in the test samples were extracted with water by means of high-speed
homogenization. The extract was derivatized with FeCl3 reagent, cleaned up by chloroform and MAX anion exchange
column. The residues were determined and confirmed by UPLC-MS-MS using an external standard method. The linear range
was 1.0–50.0 μg/mL for EDTA-2Na with a correlation coefficient (R2) of 0.992 2. The average recoveries of EDTA-2Na in
spiked samples ranged from 79.4%–105.3%, with relative standard deviations between 7.93% and 9.94% at spiked levels
of 20–400 mg/kg. The limit of detection was 20 mg/kg for EDTA-2Na. The method is sensitive, accurate, repeatable and
suitable for the determination of EDTA-2Na residues in canned eight-ingredient porridge.

Key words: ethylenediaminetetraacetic acid disodium (EDTA-2Na), ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS), canned eight-ingredient porridge