食品科学

• 基础研究 • 上一篇    下一篇

LED光源对芽期苦荞黄酮合成的影响

雒晓鹏,卜星星,赵海霞,李成磊,陈 惠,王安虎,吴 琦   

  1. 1.四川农业大学生命科学学院,四川 雅安 625014;2.西昌学院农业科学学院,四川 西昌 615000
  • 出版日期:2015-02-15 发布日期:2015-02-10

Effects of LED Lights on the Levels of Flavonoid during Germination of Tartary Buckwheat

LUO Xiaopeng, BU Xingxing, ZHAO Haixia, LI Chenglei, CHEN Hui, WANG Anhu, WU Qi   

  1. 1. College of Life Sciences, Sichuan Agricultural University, Ya’an 625014, China;
    2. School of Agricultural Sciences, Xichang College, Xichang 615000, China
  • Online:2015-02-15 Published:2015-02-10

摘要:

以暗黑萌发6 d的苦荞芽为材料,分别采用LED红光(630 nm)、LED白光(540 nm)、LED蓝光(460 nm)、LED紫外光(385 nm)和普通荧光(混合波长),以光周期为16 h/8 h(昼/夜)27 ℃处理芽期苦荞3 d。采用半定量反转录-聚合酶链式反应(semi-quantitative reverse transcription and polymerase chain reaction,RT-PCR)分析苦荞子叶和胚轴中FtPAL、Ft4CL、FtCHS、FtFLS、FtF3H和FtANS 6 个黄酮合成途径酶基因的表达量,采用AlCl3法测定不同光源处理前后的总黄酮含量,并进行相关性分析。结果表明:光源影响总黄酮在子叶中的含量大小顺序为:黑暗<荧光<白光<红光<蓝光<紫外光,光源影响总黄酮在胚轴中的含量大小顺序为:黑暗<荧光<红光<白光<蓝光<紫外光;子叶中总黄酮含量与FtPAL、FtCHS、FtF3H和FtANS的表达显著相关(相关系数>0.75),其中与FtPAL相关性最高(相关系数为0.921);胚轴中总黄酮含量仅与Ft4CL表达显著相关(相关系数为0.975)。因此,光质能够调节芽期苦荞黄酮的积累,可选用UV-B、LED蓝光和红光这3 种组合光通过优化光控条件增加黄酮含量。

关键词: LED光源, 苦荞, 芽期, 黄酮, 基因表达

Abstract:

After tartary buckwheat seeds germinated in darkness for 6 days, their sprouts were cultivated under lightemitting
diode (LED) red light, LED white light, LED blue light, LED UV-B light and fluorescent light, respectively. They
were grown under a 16 h (daytime):8 h (night) photoperiod at 27 ℃ for 3 days. The transcriptional levels of six genes
involved in the flavonoid biosynthesis pathway, FtPAL, Ft4CL, FtCHS, FtFLS, FtF3H, and FtANS, were investigated by
semi-quantitative RT-PCR. The content of total flavonoids was measured by AlCl3 method. The correlation coefficients
between total flavonoids content and the expression levels of flavonoid biosynthesis-related genes in both cotyledons and
hypocotyls were analyzed. Total flavonoids content in tartary buckwheat cotyledons with different light treatments followed
the decreasing order: UV-B > blue > red > white > florescence > dark; as for hypocotyls, the decreasing order was UV-B >
blue > white > red > florescence > dark. Total flavonoids content was significantly correlated with the expression levels
of FtPAL, FtCHS, FtF3H and FtANS in cotyledons (correlation coefficients > 0.75). While FtPAL expression showed the
highest correlation with total flavonoids content in cotyledons with correlation coefficient of 0.921, only Ft4CL expression
level was detected to have a significant correlation with total flavonoids content in hypocotyls with correlation coefficient
of 0.975. In conclusion, flavonoid accumulation during germination of tartary buckwheat could be increased through light
regulation, and UV-B, blue and red light could be mixed for induction of more flavonoids.

Key words: LED light, tartary buckwheat, germination, flavonoids, gene expression

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